朊蛋白表达对结直肠癌细胞增殖及侵袭能力的影响

Effect of cellular prionprotein expression on proliferation and invasion of colorectal cancer cells

目的 观察阮蛋白（PrPC）在结直肠癌细胞株中的表达及其对细胞增殖及侵袭能力的影响.方法 采用实时定量逆转录聚合酶链反应（RT-qPCR）及Western blot检测结直肠癌细胞株HCT-116、SW480、Lovo中PrPC的表达,然后构建prpC慢病毒小干扰RNA（siRNA）载体,转染Lovo及SW480细胞株,对照组转染空白质粒,采用噻唑蓝（MTT）法和侵袭小室实验检测PrPC表达下调后细胞的增殖及侵袭能力.结果 PrPC在Lovo及SW480细胞中的mRNA表达水平分别为8.19±1.12、7.48±1.21,明显高于HCT-116细胞（3.56±1.03）,差异有统计学意义（P＜0.05）.同时,prpC在Lovo及SW480细胞中的蛋白表达水平也高于HCT-116细胞.prpC表达下调后Lovo及SW480细胞的增殖及侵袭能力均明显下降,差异均有统计学意义（P＜0.05）.结论 PrPC表达与结直肠癌细胞的增殖及侵袭能力密切相关.

Objective To detect the effect of cellular prionprotein （PrPC） expression on proliferation and invasion of colorectal cancer cells.Methods PrPC expression was detected in HCT-116,SW480 and Lovo cell lines by real-time reverse transcriptase-polymerase chain reaction （RT-qPCR） and Western blot.After transfecting the Lovo and SW480 cell lines by PrPC spefical lentivirus vectors,the ability of proliferation and invasion was evaluated by methyl thiazol tetrazolium （MTT） assay and Transwell assay,respectively.Results The mRNA level of PrPC was higher in Lovo and SW480 cell lines （8.19 ± 1.12,7.48 ± 1.21,respectively） compared with those in HCT-116 cell line （3.56 ± 1.03）.After downregulation of PrPC,cell proliferation and invasion were significantly inhibited in Lovo and SW480 cell lines,with a significant difference （P 〈 0.05）.Conclusion PrPC expression is closely associated with the proliferation and invasion of colorectal cancer cells.