摘要
目的 检测上皮钙黏蛋白（E-cadherin）和锌指转录抑制因子Snail在肝细胞癌（HCC）组织中的表达,探讨其在HCC发生发展中的意义.方法 采用实时定量聚合酶链反应（Real-timePCR）法检测36对HCC及癌旁组织中的E-cadherin和Snail mRNA表达,分析两者在HCC中的表达意义及相关性.结果 E-cadherin mRNA在HCC中的表达水平明显低于癌旁组织（0.41±0.18比1.00±0.14,P＜0.01）,而Snail mRNA在HCC中的表达水平明显高于癌旁组织（1.78±0.34比1.00±0.25,P＜0.01）;两者均与HCC的肝内转移、包膜是否完整、血管侵犯及分化程度密切相关（P＜0.05）;两者在HCC中的表达呈负相关（r=-0.835,P＜0.01）.结论 E-cadherin和Snail与HCC的侵袭和转移密切相关,可能在HCC侵袭和转移中发挥重要作用.
Objective To detect the expression of E-cadherin and Snail in hepatocellular carcinoma （HCC） and investigate their roles in HCC genesis and development.Methods The expression levels of E-cadherin and Snail mRNA were detected by using real-time quantitative polymerase chain reaction （Real-time PCR） in 36 pairs of HCC and adjacent non-cancerous tissues.The significance and correlation of them in HCC were analyzed.Results E-cadherin mRNA expression was significantly down-regulated in HCC tissues as compared with matched adjacent non-cancerous tissues （0.41 ±0.18 vs.1.00 ± 0.14,P 〈0.01）,and Snail mRNA expression was significantly higher in HCC tissues than in matched adjacent non-cancerous tissues （1.78 ±0.34 vs.1.00 ±0.25,P 〈0.01）.The expression levels of E-cadherin and Snail mRNA were significantly correlated with intrahepatic metastasis,capsular invasion,vascular invasion and tumor grade in HCC （P 〈 0.05）.There was a negative correlation between the expression of E-cadherin and Snail in HCC （r =-0.835,P 〈 0.01）.Conclusion E-cadherin and Snail were significantly correlated with invasion and metastasis of HCC.E-cadherin and Snail likely play important roles in invasion and metastasis of HCC.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2014年第2期298-300,F0003,共4页
Chinese Journal of Experimental Surgery
基金
国家自然科学基金资助项目（81201930）
高等学校博士学科点专项科研基金项目（新教师类）（20124423120006）
广东省自然科学基金资助项目（S2012040006803）
广州市科信局重点资助项目（2011J4100053）
广州医学院博士启动基金资助项目（2010C23）
