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大鼠脊髓血供网络的同步辐射类同轴相位衬度成像 被引量:1

Visualization of blood supply network using X-ray in-line phase contrast imaging in rat spinal cord
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摘要 目的 应用同步辐射类同轴相位衬度成像技术(IL-XPCI)重现大鼠脊髓微血管的二维和三维结构,并建立稳定性和重复性良好的成像方法.方法 取SD雄性大鼠6只,脊髓标本在不行血管造影的条件下,经灌注固定、梯度脱水后于上海光源X射线成像与生物医学应用光束线站进行扫描和成像,观察大鼠脊髓微血管成像效果.结果 大鼠脊髓微血管的相位衬度信息表现为显著的边缘增强效应,在无造影的条件下,同步辐射类同轴相位衬度成像技术成功再现了脊髓血供的三维网络结构,可分辨最细血管直径达7.4 μm左右.结论 同步辐射类同轴相位衬度成像技术在血管相关脊髓疾病的研究中具有广阔的应用前景. Objective To investigate the two-dimensional and three-dimensional micro-vascular atlas of rat spinal cord using synchrotron radiation-based in-line X-ray phase contrast imaging (XPCI),and proceed to establish steady and repeatable imaging method.Methods Six Sprague-Dawley male rats were used in this study.The spinal cord specimens were subjected to fixation and dehydrated without angiography,followed by the scanning and imaging procedure in X-ray imaging and bio-medical applications beam line station in Shanghai Synchrotron Radiation Facility.The results and effects of XPCI concerning microvasculature of rat spinal cord were evaluated.Results The phase contrast signal of vessels in rat spinal cord was characterized by the significant effect of edge enhancement in XPCI imaging.The 3D morphology of the rat spinal cord microvasculature could be successfully observed via the IL-XPCI without the contrast agent injection and the smallest vessel with diameter of about 7.4 μm could be visualized.Conclusion XPCI has broad applied value in vascular related spinal cord diseases.
出处 《中华实验外科杂志》 CAS CSCD 北大核心 2014年第2期392-394,共3页 Chinese Journal of Experimental Surgery
关键词 同步辐射 类同轴相位衬度成像 脊髓 微血管 形态学 Synchrotron radiation Phase contrast imaging Spinal cord Microvasculature Morphology
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