人端粒酶逆转录酶mRNA在胆囊癌患者外周血中的表达

Expression of human telomerase reverse transcriptase mRNA in the peripheral blood of gallbladder carcinoma patients

目的 探讨人端粒酶逆转录酶mRNA（hTERT） mRNA在胆囊癌患者外周血中的表达及临床意义.方法 选择50例胆囊癌（胆囊癌组）、50例腺瘤性息肉（腺瘤性息肉组）患者,另选取50例正常体检者（对照组）,采用实时荧光定量聚合酶链反应（FQ-PCR）检测外周血hTERT mRNA.结果 胆囊癌组hTERT mRNA阳性率（66％）明显高于腺瘤性息肉组（14％,P＜0.05）,腺瘤性息肉组阳性率（14％）高于对照组（0,P＜0.05）,hTERT mRNA在T3＋T4与T1＋T2间及淋巴结阳性、阴性间差异有统计学意义（P＜0.05）.结论 胆囊癌外周血hTERT mRNA阳性率明显增高,与胆囊癌浸润深度与有、无淋巴结转移有关.

Objective To study the human telomerase reverse transcriptase （hTERT） mRNA in peripheral blood of patients with gallbladder carcinoma and its clinical significance.Methods Fifty cases of gallbladder cancer （GBC group）,50 cases of adenomatous polyps （AMP group） and 50 normal volunteers （control group） were selected.The expression of hTERT mRNA in peripheral blood was detected by using real-time fluorescent quantitative polymerase chain reaction （FQ-PCR）.Results The positive rate of hTERT mRNA in GBC group （66%） was significantly higher than in AMP group （14%） （P 〈 0.05）,and that in AMP group was higher than in control group （P 〈 0.05）.There was significant difference in the hTERT mRNA expression between T3 ＋ T4 and T1 ＋ T2 or between negative and positive lymph nodes （P 〈 0.05）.Conclusion The hTERT mRNA expression in peripheral blood of GBC was significantly increased,and associated with depth of invasion and lymph node metastasis.