乳腺癌组织中Livin蛋白的表达及其与患者临床病理特征和细胞增殖的关系

Expression of Livin protein in breast cancer tissue and its relationship with clinical pathological characteristics and cell proliferation of patients

目的探讨乳腺癌组织中Livin蛋白的表达及其与患者临床病理特征和细胞增殖的关系。方法采用免疫组化S-P法检测乳腺癌组织中Livin蛋白表达,并探讨其与患者年龄、组织学分级、临床分期和淋巴结转移等临床病理特征和细胞增殖的关系。另选择20例癌旁正常组织和10例正常乳腺组织作对照。结果 Livin蛋白在正常乳腺、癌旁正常组织及乳腺癌组织中的表达呈逐渐增高趋势(χ2=18.28,P<0.01)。乳腺癌组织中Livin蛋白表达与临床分期和淋巴结转移密切相关(χ2=4.19和7.73,P<0.05或P<0.01)。Livin表达阴性乳腺癌组织中PCNA增殖指数明显低于Livin表达阳性乳腺癌组织(t=2.37,P<0.05)。结论 Livin蛋白在乳腺癌发生和发展不同阶段的表达呈进行性上升趋势,Livin蛋白在乳腺癌细胞的增殖、侵袭、转移等活动中可起到促进作用,可作为预后评估的指标。

Objective To discuss expression of Livin protein in breast cancer tissue and its relationship with clinical pathological characteristics and cell proliferation of patients. Methods The expression of Livin protein in breast cancer tissue was detected by immunohistochemical S-P method, and its relationship with clinical pathological characteristics of patients such as ages of patients, histological grade, lymphatic metastasis and etc and cell proliferation was dis- cussed. Additionally, 20 cases of normal tissues adjacent to cancer and 10 cases of normal mammary tissue were selected as control subjects. Results The expression of Livin protein in normal breast, normal tissues adjacent to cancer and breast cancer tissue showed the gradually increasing trend （χ2=18.28 ,P〈0.01 ）. The expression of Livin protein in breast cancer tissue had close effect on clinical stages and lymphatic metastasis（χ2=4.19 and 7.73, P〈0.05 or P〈0.01 ）. The proliferation index of PCNA in breast cancer tissues, in which the Livin shows the negative expression, is much lower than that in breast cancer tissues, in which the Livin shows the active expression （t=2.37,P〈0.05）. Conclusion The expression of Livin protein in different stages of occurrence and development shows the progressive upward trend, and the Livin protein plays a promoting role in the activities of invasion and transfer of breast cancer cells, which can be the index of evaluation of prognosis.