胀果甘草的简便快速鉴定方法

Rapid and simple identification of Glycyrrhiza Inflata

目的研究胀果甘草(Glycyrrhiza inflata Bat)的快速鉴定方法。方法利用超声辅助的乙醇提取法制备新疆胀果甘草和光果甘草的总黄酮类提取物;利用硅胶薄层色谱法分析2种甘草总黄酮提取物的斑点特征;利用制备薄层法分离薄层板上的特殊斑点并对所分离的单体成分进行结构分析。结果胀果甘草乙醇提取物在展开剂为乙酸乙酯∶甲酸∶水(8∶3∶1)的薄层色谱条件下所显示的一种特殊亮黄色斑点被鉴定为甘草查尔酮A,色谱特征不同于其他甘草品种。结论以甘草查尔酮A为对照品的硅胶搏层色谱法是快速鉴别胀果甘草的简便方法,适合于甘草品种的快速鉴定及资源考察研究。

Objective To study the rapid and simple identification method of the licorice specieGlycyrrhiza inflata Bat.Methods The total flavonoid extracts of two licorice species Glycyrrhiza inflata Bat （GIB） and Glycyrrhiza glabra L （GGL）were prepared by ultrasonic assisted ethanol extraction;the thin layer chromatographic （TLC）method was used for analyzing the spectra characteristics of these extracts;the special TLC spot was isolated and structurally analyzed.Results The TLC characteristics of two species of licorice indicated that the GIB extract showed,at the mobile phase of acetic acetate/methyl acetate/water （8∶3∶1）,a special spot and was identified as Licochalcone A （LicoA）,which was different form other species of licorice.Conclusion The silica gel chromatographic method with LicoA as the reference sub-stance was simple and suitable for the rapid identification of glycyrrhiza inflata from other licorice spe-cies,as well as for the licorice resource study and licorice quality control.