Nestin和Ki67在卵巢上皮性癌组织中的表达及临床意义

Expression and clinical significance of Nestin and Ki67 in epithelial ovarian cancer

目的 探讨Nestin及Ki67在卵巢上皮性癌组织中的表达及其与临床病理特征的关系.方法 对2007年1月～2009年1月邯郸市中心医院妇科60例卵巢癌患者及20例卵巢正常患者的病理资料进行回顾性分析,采用免疫组化SP法检测卵巢上皮性癌标本及正常组织中Nestin及Ki67的表达,分析并比较正常卵巢组织及不同病理类型、FIGO分期、残余肿瘤直径的卵巢组织中Nestin及Ki67的表达情况.结果 ①卵巢上皮性癌标本中Nestin和Ki67阳性表达率（38.5％、76.7％）明显高于正常卵巢组织（5.0％、15.0％）,两者相比,差异均有统计学意义（均P＜0.05）.②组织分化程度高级别的卵巢癌组织中Nestin及Ki67阳性表达率明显高于低级别者（P＜ 0.05）;FIGO分期越高、残余肿瘤直径越大,Nestin、Ki67阳性表达率越高（均P＜0.05）.③Nestin与Ki67在卵巢癌中的表达无明显相关性（r=0.176,P=0.13）.结论 Nestin与Ki67的表达与卵巢癌的发生、发展有密切关系,对Nestin与Ki67的检测可用于判断卵巢上皮性肿瘤的恶性程度及其预后.

Objective To investigate the expression of Nestin and Ki67 in epithelial ovarian cancer and the relationship between their expression and clinicopathologic characteristics. Methods pathological files of 60 patients with epithelial ovarian carcinoma and 20 patients with nomal ovarian tissues from January 2007 to January 2009 in Gynecology De- partment of Central Hospital of Handan City were retrospective analyzed, the Nestin and Ki67 were detected by im- munohistochemistry （SP method）, the expression of Nestin and Ki67 in nomal ovarian and epithelial ovarian cancer tissues with different pathological pattern, FIGO stages and residual tumor size were analyzed and compared. Results Fhe positive expression rate of Nestin Ki67 in ovarian epithelial carcinomas （38.5%, 76.7%） were higher than those in nor mal ovarian tissues（5.0% , 15.0%）, the differences were statistically significant（all P 〈 0.05）. The positive expression of Nestin and Ki67 in epithelial ovarian cancer tissues with high differentiation degree was higher than those in with low differentiation degree （all P 〈 0.05）. The higher FIGO stage and larger residual tumor size, the higher positive ex pression of Nestin and Ki67 in epithelial ovarian cancer tissues. There was no significant correlation between Nestin and Ki67 expression （r = 0.176, P = 0.13）. Conclusion The expressions of Nestin and Ki67 are associated with the de velopment and progression of the ovarian epithelial neoplasm, the detection of Nestin and Ki67 may be helpful to esti mate the malignant degree and prognosis of the epithelial ovarian carcinoma.