摘要
根据细菌基因组内广泛存在的短重复序列ERIC设计引物,采用Rep-PCR技术对来自广东省主要红掌种植的9个市区27个品种的66个红掌细菌性疫病菌株(Xanthomonas axonopodis pv.dieffenbachiae,简称Xad)进行遗传多样性分析。结果显示,在相似水平为0.85时,可以分为14个遗传组,其中Ⅰ、Ⅱ和Ⅹ为主要遗传组,这表明广东红掌细菌性疫病菌遗传分化明显且具多样性。
The evaluation of genetic diversity of 66 Xanthomonas axonopodis pv. dieffenbachiae （Xad） strains from 27 anthurium varieties in 9 cities from Guangdong Province was conducted by Rep- PCR technology. The DNA of Xad was amplified by the primer ERIC and the genetic diversity was evaluated by UPGMA clustering analysis. The results showed that 66 Xad strains could be divided into 14 genetic groups at the similarity level of 0.85, and the genetic group Ⅰ , Ⅱ and Ⅹ were the major groups. It shows that there were obvious differentiation and rich diversity in X. axonopodis pv. dieffen- bachiae strains in Guangdong Province.
出处
《华中农业大学学报》
CAS
CSCD
北大核心
2014年第2期40-45,共6页
Journal of Huazhong Agricultural University
基金
广东省科技计划项目(2010B020401002)
广州市科技计划项目(4200-10028)
关键词
红掌
细菌性疫病菌
遗传多样性
REP-PCR
Anthuriurn andraeanurn
Xanthornonas axonopodis pv. dieffenbachiae
genetic diversi- ty
Rep-PCR
