乌骨山羊皮肤黑色素细胞的分布及特征

Location and characteristics of melanocytes of black-boned goat skin

试验采用免疫组织化学染色对乌骨山羊不同被毛颜色皮肤中的黑色素细胞进行定位,再用多巴组织化学染色法对皮肤中有黑色素分泌的黑色素细胞进行定位,以HE染色法对黑色素细胞的形态进行观察。免疫组织化学染色结果显示,乌骨山羊皮肤中的黑色素细胞主要分布在表皮基底层和毛囊中;多巴组织化学染色结果显示,表皮中没有阳性反应,黑色被毛毛球部及外根鞘均有阳性反应,白色被毛毛球部及外根鞘则没有阳性反应,说明黑色被毛毛球部及外根鞘有成熟的黑色素细胞存在并能合成黑色素。HE染色结果显示,黑色被毛毛囊外毛根鞘中的黑色素细胞和周围细胞区分明显,呈空泡状和活化状态,周围有大量黑色素颗粒分布,白色被毛毛囊外根鞘黑色素细胞呈椭圆状,没有黑色素产生,和周围细胞难以区分。因此,乌骨山羊黑色被毛毛囊外根鞘中黑色素细胞的活化可能参与了乌骨山羊毛色和乌质性状的形成。

This paper focused on location and characteristics of melanocyte in skin of black-boned goat, providing clues for melanin deposition research. By immunohistochemistry staining, the localization of melanocytes in the black-boned goat skin with different coat colors （black and white） was examined. The mature melanocytes were determined by DOPA staining. The morphology of melanocytes was ana- lyzed using hematoxylin-eosin（HE） staining. The immunohistochemistry results showed that melano- cytes were mainly located in basal layer of epidermis and hair follicles ~ The DOPA staining revealed that melanocytes were not identifiable in basal layer of the two coat colors epidermis and white hair follicles but existed in the bulb of black hair follicles and outer root sheath. It turned out that there were mature melanocytes existing in the bulb of black hair follicles and outer root sheath, which could synthesize mel- anin. The hematoxylin-eosin （HE） staining results suggested that melanocytes in the outer root sheath of black hair follicles had intense pigmentation and a tendency for vacuolization while melanocytes in white hair follicles were elliptical shape and produced no melanin. What＇s more we can＇t distinguish the melanocytes from the other surrounding cells. Therefore, the activation of the melanocytes in the outer root sheath of black hair follicles might be the main reason for the melanin trait of black-boned goat.