枇杷少核株系花粉管生长差异研究

Research on the Difference in the Pollen Tube Growth of a Low Seediness Line of Loquat

以‘大五星’枇杷退化种子少核株系‘川农1号’(C1)为试材,以‘大五星’枇杷自花授粉为对照,采用荧光显微技术,对枇杷少核株系自花、异花授粉后花粉管生长情况进行观察。结果表明:(1)C1株系异花授粉后花粉能在柱头上萌发,并伸入中央花柱道,在授粉后48h左右到达花柱基部。(2)C1株系自花授粉后,花粉萌发与花粉管的伸长速度相对于异花授粉滞后,自花授粉后36h大多数花粉管到达花柱的中上部并停止生长,且伴随产生花粉管顶端形态异常、荧光异常明亮等现象,最终只有极少数花粉管能到达花柱基部。研究表明,C1株系为配子体自交不亲和,C1株系的自交不亲和性是引起其少核的重要原因之一。

In this test, ＇ Chuannong 1 Ioquat＇ （C1） was taken as the experimental material which was derived from a low seediness line of degenerated seeds of＇Dawuxing＇ loquat. The self-pollination of ＇Dawuxing＇ loquat was the control treatment. The growth of pollen tubes in the styles was observed with fluorescence microscopy. The results showed that. （1） Pollens germination and pollen tube growth were normal in cross-pollination of C1 and it took about 48 hours for pollen tubes to reach the base of style. （2）The pollens could germinate on stigma and the pollen tubes could growth in the styles after self-pollination of C1, but it took quite a long time. Most pollen tubes could reach the upper or middle of style but stopped growing 36 hours after self-pollination of C1, and meanwhile there were some incompatibility-phenomenon that the form of tube apex was abnormal and fluoresced more obviously, and only few pollen tubes could reach the base of style. C1 behaved gametophytic self-incompatibility （GSI）. The self-incompatibility of C1 was one of the important reasons for its seed abortion.