腹腔注射氯胺酮建立膀胱炎模型及其尿流动力学指标评价

Establishment of ketamine-associated cystitis rats model and method of urodynamic studies

目的探讨氯胺酮性膀胱炎大鼠模型的建立方法,为研究氯胺酮性膀胱炎提供可靠的动物模型。方法将30只雌性SD大鼠随机分为两组,对照组10只,氯胺酮组20只。模型组予氯胺酮30 mg·kg^(-1)腹腔注射,每日1次,共12周。对照组注射等容量生理盐水。检测两组大鼠排尿间隔时间、最大膀胱容量(MBC)、最大排尿压(MVP)和逼尿肌不稳定收缩频率,并比较膀胱病理学差异。结果与给药前比较,给药后氯胺酮组大鼠排尿闾隔时间、MBC随时间进行性下降(P<0.05),逼尿肌不稳定收缩频率进行性增高(P<0.05),MVP无明显变化(P>0.05)。12周后,对照组大鼠排尿间隔时间和MBC均高于氯胺酮组(P<0.01),未测得逼尿肌不稳定收缩频率。与对照组比较,氯胺酮组大鼠膀胱黏膜上皮显著增厚,黏膜下层腺管增粗,腺腔增多、增大,有炎症细胞侵润。结论腹腔注射氯胺酮可以诱导大鼠患氯胺酮性膀胱炎,并可作为研究氯胺酮性膀胱炎的可靠模型。

AIM To explore the method of establishment the rat model which can be used as a reliable animal model for the study of ketamine cystitis. METHODS Thirty female SD rats were randomly divided into two groups and which contained control group （10 rats） and ketamine group （20 rats）. The rats in the model group were treated daily by intraperitoneal injection of ketamine 30 mg ＂kg-~ and the equal volume of normal saline were given to rats in the control group, both lasted 12 weeks. The voiding interval, maximum bladder capacity （MBC）, maximum voiding pressure （MVP）, unstable contraction frequency of detrusor and pathology of bladder were observed. RESULTS The voiding interval, MBC reduced progressively in the ketamine group （P 〈 0.05）, while the unstable contraction frequency of detrusor increased （P 〈 0.05）. There was no obvious change in MVP in the ketamine group. After 12 weeks, the levels of voiding interval and MBC in the control group were higher than those in the ketamine group （P 〈 0.01 ） , and the unstable contraction frequency of detrusor wasn＇ t detected. Compared with the control group, ketamine model rats showed a thicker and more compact epithelial layer and inflammatory cell infiltration in the sub- mucosal layer. CONCLUSION Intraperitoneal injection of ketamine can induce to ketamine cystitis which can be used as a reliable animal model for the study of ketamine cystitis.