摘要
华蟾素为中华大蟾蜍皮水煮醇沉制剂,临床具显著抗肿瘤活性。本文首次采用高效液相色谱串联高分辨傅立叶变换离子回旋共振质谱(HPLC-DAD-FT-ICR-MS)方法,针对华蟾素中主要抗肿瘤活性成分蟾毒配基类化合物进行了分析,旨在阐明华蟾素抗肿瘤活性效应物质基础。该研究首先采用二氯甲烷-硅胶固相萃取法对华蟾素中蟾毒配基类成分进行富集,根据化合物的UV及FT-ICR-MS高分辨质谱的一级、二级质谱数据,结合文献,比较分析了华蟾素中的主要蟾毒配基类成分的化学组成,共推导出33个蟾毒配基类成分,其中8个成分通过与对照品比对,准确鉴定了结构。该研究基本阐明了华蟾素中主要蟾毒配基类成分,为华蟾素的进一步药效学研究奠定了物质基础。
Cinobufacino injection is a significant anti-tumor medicine for the treatment of various tumors in clinic, which was made from water extraction of the skin of Bufo bufo gargarizans. In present paper, HPLC- DAD-FT-ICR-MS method was used to identify the major bufadienolides in cinobufacino for the first time. Solid-phase extraction with dichloromethane and silica was used to enrich the total bufadienolides in cinobufacino. Based on the UV and high resolution MS/MS data, 33 bufadienolides were analyzed and characterized. Among them, eight compounds were identified by comparing with standard references unambiguously. This study elucidated the major bufadienolides in cinobufacino, which provided material foundation of cinobufacino and will be benefit for the further pharmacological research.
出处
《药学学报》
CAS
CSCD
北大核心
2014年第2期244-248,共5页
Acta Pharmaceutica Sinica
基金
科技部重大新药创制专项资助项目(2009ZX09103-404)
