体外模拟间质液压升高对涎腺腺样囊性癌细胞增殖和侵袭能力的影响

Increased invasion ability mechanism of salivary adenoid cystic carcinoma through elevated interstitial fluid pressure in vitro

目的模拟肿瘤内间质液压(IFP)的施加情况,建立腺样囊性癌细胞受力模型,研究不同IFP作用下腺样囊性癌细胞ACC-2的增殖和侵袭能力的变化。方法将压力锅改装成能稳定调控气压的数控力学加压装置。体外培养ACC-2细胞,以不同的压力(7.551、7.649、7.747 kPa)处理细胞并持续不同的时间(3、6、12、24 h),以未加压的ACC-2细胞为阴性对照。用CCK-8法测定细胞的生长情况,并检测细胞Ki67的表达情况以研究细胞的增殖能力;用Transwell小室试验观察细胞的侵袭能力。结果在7.649 kPa下加压6 h后细胞的增殖能力增强,并随着加压时间的延长逐渐增强,并在加压24 h后达到最大值;细胞在加压后的侵袭能力明显提高,并在加压24 h后达到最强。结论成功建立了腺样囊性癌细胞受力模型;IFP的升高可刺激细胞增殖,提高其侵袭能力。

Objective Through a simulation of interstitial fluid pressure （IFP）, we developed an in vitro model to explore the change law of biological characteristics of adenoid cystic carcinoma （ACC） under different IFP. Methods A pressure cooker was refitted into a controllable pressure device. Cultured ACC-2 cells were subdivided into different groups, namely, negative control （untreated ACC-2） and experimental group （stressed for 3, 6, 12, 24 h under pressure of 7.551, 7.649, 7.747 kPa）. CCK-8 and immunofluorescence of Ki67 were used to reflect proliferation ability. Transwell chamber assay was performed to observe the invasion ability of cells. Results The proliferation ability was positively correlated with treatment time, and the peak value was obtained after the cells were subjected to 7.649 kPa of stress for 24 h. The invasion ability of ACC-2 cells was upregulated under stress. Conclusion We successfully developed an in vitro model of IFP and found that high IFP can stimulate cell proliferation ability and upregulate invasion ability.