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H4N2亚型禽流感病毒反向遗传操作系统的构建 被引量：2

Establishment of reverse genetics system for H4N2 avian influenza virus
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摘要 为建立H4N2亚型禽流感病毒(AIV)反向遗传操作系统,本研究构建了A/Duck/JiangXi/21046/2012(H4N2)(W-DKJX)的8个重组质粒,拯救出A/Duck/JiangXi/21046/2012(H4N2)(R-DKJX)。全基因组序列测序结果表明,拯救病毒R-DKJX与野生病毒W-DKJX的核苷酸序列完全相同。受体结合试验表明,野生病毒与拯救病毒均具有人型受体结合特性。以106EID50/50μL剂量鼻腔感染BALB/c小鼠,病毒均仅在小鼠的呼吸道复制,对BALB/c小鼠呈低致病性,可以在感染早期引起小鼠体重轻微下降但并未引起发病或死亡。以上结果表明,两者保持了一致的生物学特性。H4N2亚型AIV反向遗传操作系统的构建为进一步研究H4亚型AIV的致病机理及其跨种传播分子机制等提供了技术平台。 To establish an eight-plasmid reverse genetics system of H4N2 subtype avian influenza virus, a strain of A/Duck/JiangXi/21046/2012 （W-DKJX） was rescued, named R-DKJX. Compared with W-DKJX, R-DKJX had the same human-type receptor binding specificity. It was restricted in the respiratory system of mice after inoculated intranasally with 106 EID50/50 μL of virus. The virus was low pathogenic to mice and only caused light weight loss at early infection, but no illness or death. In conclusion, the rescued R-DKJX had the same biological characteristics with the widetype virus W-DKJX. The H4N2 subtype avian influenza virus reverse genetics system could provide a promising technique support for further study on the pathogenic and trans-species transmission mechanisms of theH4 avian influenza virus.
作者 王帅 梁立滨 邓国华 施建忠 姜永萍 关云涛 陈化兰
机构地区 中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室/农业部动物流感重点开放实验室
出处 《中国预防兽医学报》 CAS CSCD 北大核心 2014年第2期90-93,共4页 Chinese Journal of Preventive Veterinary Medicine
基金 农业部专项(CARS-42-G08)
关键词 H4N2 禽流感病毒 反向遗传操作系统 H4N2 avian influenza viruses reverse genetics system
分类号 S852.65 [农业科学—基础兽医学]
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参考文献13

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中国预防兽医学报
2014年 第2期

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