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猪博卡病毒全基因组序列分析与基因分型研究 被引量：15

Analysis of complete genomic characterization and the genotyping classification of porcine Bocaviruses
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摘要 为了解猪博卡病毒(PBoV)的基因组结构和遗传进化规律,并研究可行的PBoV基因分型方法,本研究利用GenBank中登录的28条PBoV全基因组序列信息,采用DNAStar生物信息学软件进行基因组序列分析。结果显示,分别选取全基因组、NS1基因、NP1基因和VP1基因构建的系统进化树,其基因分型结果基本一致,PBoV均被划分为3个基因群,基因群内各病毒株的核苷酸序列同源性较高,并且具有独特的序列特征和规律,而各基因群之间病毒株的核苷酸序列同源性非常低,基因组结构差异较大,充分证明了PBoV基因分型的可行性。 To analyze the genomic characterization and classify the genotypes of porcine Bocavirus （PBoV）, the complete genomic sequences of 28 PBoVs available in GenBank were analyzed for the homology of NS 1, NP1, VP1 and complete genomic sequences by sotb0vare of DNAStar. The results showed that PBoV could be divided into three groups based on phylogenetic tree established according to PBoV NS1, NP1, VP1 and complete genomic sequences, of which in the same group shared higher sequence identity, otherwise, these sequences in different groups shared lower sequence identity.
作者 覃绍敏 吴健敏 马琳 袁龙 陈凤莲 马玲 白安斌
机构地区 广西兽医研究所 广西动物疫病预防控制中心 吉林大学动物科学学院
出处 《中国预防兽医学报》 CAS CSCD 北大核心 2014年第2期150-153,共4页 Chinese Journal of Preventive Veterinary Medicine
基金 广西科技攻关与新产品试制项目(桂科攻1123007) 广西水产畜牧兽医局科技计划项目(桂渔牧科1304524)
关键词 猪博卡病毒 遗传进化分析 基因分型 porcine Bocavirus phylogenetic analysis genotyping
分类号 S852.65 [农业科学—基础兽医学]
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参考文献14

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二级参考文献8

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共引文献19

同被引文献92

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  • 3陈鹏强,胡崇伟,陈秋勇,林群群,修金生.猪博卡病毒5型SYBR GreenⅠ实时荧光定量PCR检测方法的建立[J].中国兽医科学,2015,45(2):150-155. 被引量：5
  • 4刘丽娜,何启盖,陈焕春,刘正飞,张松林,汪招雄.用多重PCR鉴别猪伪狂犬病野毒与疫苗毒的研究[J].中国兽医科技,2005,35(2):95-98. 被引量：38
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引证文献15

二级引证文献24

中国预防兽医学报
2014年 第2期

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