脂蛋白基因在早期骨关节炎软骨下骨的表达

Expression of lipoprotein related genes in subchondral bone of early experimental osteoarthritis

目的:研究早期骨关节炎软骨下骨脂蛋白相关的基因表达改变情况。方法:大鼠分为实验组(15只)和对照组(15只)。实验组切除右膝内侧半月板及内侧副韧带,对照组仅切开关节囊。于术后1、2、4周取右膝关节标本,采用全基因表达谱芯片技术研究软骨下骨全基因表达,利用差异基因分析的方法分析脂蛋白相关的基因表达改变情况。结果:软骨下骨Apoa5表达于建模术后1周上调,术后2周下调;Apoc2表达于术后2周上调;Apol3表达于术后1周上调,术后4周下调;Lrp1于术后1、2周下调;Lrp5于术后2周下调;Gpihbp1、Lpl、Tfpi、Vldlr表达于术后1周均上调;Lrpap1、RGD1309808于术后4周均下调。结论:脂蛋白相关基因的表达改变在早期膝关节骨关节炎的软骨下骨变化中可能起了重要作用。

Objective： To study the expression of lipoprotein related genes in subchondral bone of early experimental osteoarthritis,which may play an important role in the pathogenesis of osteoarthritis. Methods： Animals are equally divided into two groups：experimental group and control group,both of which contain fifteen rats of similar weight. The right knee joints of experimental group underwent surgery,which involved in both medial collateral ligament（MCL） transaction and medial meniscectomy,while the control group was only carried out with a sham operation. Rats were killed at 1,2 and 4 weeks postsurgery to obtain the right knee joints. Total RNA of the subchondral bone was extracted,and then hybridized to Agilent Whole Rat Genome Microarray. Differentially expressed genes analysis was used to study the chemokine signaling pathway. Results： Apoa5 expression was down-regulated at 1,2 weeks post-surgery,Apoc2 expression was up-regulated at 1 week after surgery,Apol3 expression was up-regulated at 1 and down-regulated at 4 weeks post-surgery,Lrp1 expression was down-regulated at 1,2 weeks after surgery. Lrp5 was down-regulated at 2 weeks after surgery. Gpihbp1,Lpl,Tfpi and Vldlr were up-regulated at 1 weeks after surgery. Lrpap1 and RGD1309808 were down-regulated at 4 weeks after surgery. Conclusion： Dysregulation of lipoprotein related genes plays an important role in pathogenesis of early experimental osteoarthritis.