咖啡因对大鼠海马神经元形态学的影响

Effect of caffeine on neuroethology of hippocampal neurons of rats

目的观察不同剂量咖啡因对大鼠海马神经元形态结构的影响。方法 30只雌性SD大鼠随机分成对照组、低剂量组和高剂量组,每天分别给予生理盐水、20mg/kg和60mg/kg咖啡因灌胃处理,连续18d。运用HE染色、尼氏染色和电镜进行观察。结果HE染色未见明显异常;尼氏染色显示,实验组尼氏体较对照组显著增多(P<0.05),高剂量组尼氏体较低剂量组有所减少;电镜观察发现,实验组海马神经元内游离核糖体密度较对照组明显增高,高剂量组海马部分神经元局部内质网轻度扩张、线粒体肿胀、神经髓鞘松懈受损。结论咖啡因能够使大鼠尼氏体数量增加,但过高剂量会造成海马神经元超微结构损坏。

Objective To observe the effect of caffeine on the morphological structure of the hippocampal neurons of rats. Methods Thirty female SD rats were randomly devided into control group, low-dose group and high-dose group, and given a gavage of normal saline, 20mg/kg and 60mg/kg caffeine for 18 days respectively. HE staining, Nissl staining and transmission electron microscopy（TEM） were used. Results Nissl staining showed that the number of Nissl body increased significantly in caffeine groups than in the normal contol（ P 〈0.05）, but with no change for HE staining. TEM revealed that the density of free ribosomes elevated dramatically in caffeine groups than in control group, with minor expansion of partial endoplasmic reticulua, swollen mitochondrial and slightly loose sheath of nerve fibers in some hippocampal neurons of high-dose group. Conclusion Caffeine could increase the number of Nissl body of the hippocampal neurons, but high dose of caffeine could damage the ultrastructure of hippocampal neurons.