活脉通络饮对大鼠脑缺血再灌注脑组织NF-κB和IL-1β表达的影响

The effect of Huomaitongluo decoction on NF-κB and IL-1β expression in cerebral ischemia and reperfusion rats

目的观察活脉通络饮对脑缺血再灌注大鼠脑组织核转录因子-κB(NF-κB)和白细胞介素-1β(IL-1β)蛋白表达的影响,探讨活脉通络饮防治脑缺血的作用机制。方法采用改良的线栓法,建立大鼠大脑中动脉栓塞(MCAO)脑缺血再灌注模型。分别设正常对照组、缺血再灌注组、活脉通络饮低剂量组、活脉通络饮中剂量组、活脉通络饮高剂量组和银杏叶片阳性对照组。分别于脑缺血再灌注术后24h后开始灌胃给药,每d给药2次,连续3 d。采用Western Blot定性和定量法检测脑组织中NF-κB和IL-1β蛋白的表达。结果与对照组相比,缺血再灌注组大鼠脑组织NF-κB和IL-1β蛋白表达增强(P<0.01);与缺血再灌注组比较,活脉通络饮低剂量组和中剂量组及银杏叶片阳性对照组大鼠脑组织NF-κB和IL-1β蛋白表达明显减少(P<0.01或P<0.05),而活脉通络饮高剂量组与缺血再灌注组相比没有统计学差异。结论活脉通络饮对脑缺血再灌注脑损伤的治疗作用可能与其下调大鼠脑组织NF-κB和IL-1β蛋白表达相关。

Objective To observe the Huomaitongluo decoction on nuclear factor-g B（NF-κB） and interleukin-1β （IL-1β ） proteins expression in cerebral ischemia and reperfusion rat brain, and to explore the protective mechanism of the Huomaitongluo decoction. Methods The rat middle cerebral artery occlusion （MCAO） model of cerebral ischemia and reperfusion was established by modified suture method. The rats were devided into normal control group, ischemia-reperfusion group, Huomaitongluo decoction low-dose group and the middle dose group and high-dose group, and Ginkgobiloba positive control group respectively. Huomaitongluo decoction and Ginkgobiloba were administered orally 24h after reperfusion, two times a day for three consecutive days. Western blot was used to detect the expression of NF-κB and IL-1β proteins. Results The expression levels of NF- κB and IL-1β proteins in brain tissue of rat were increased in ischemia-reperfusion group than in control group（ P 〈0.01）, but lower in Huumai tongluo decoction low-dose group and middle dose group and ginkgobiloba group than in ischemia-reperfusion group（ P 〈0.01 or P 〈0.05）, but with no significant difference between the high dose group and ischemia-reperfusion group. Conclusion The treatment effect of Huomaitongluo decoction on the ischemia-reperfusion damage might be related to the downexpression of NF- κB and IL- 1β in brain tissue.