摘要
RAW264.7细胞分别用阳性药地塞米松和青天葵黄酮F(nervilifordin F,从毛唇芋兰中提取得到)处理后,再用脂多糖(LPS)刺激2 h诱导炎症反应。采用Griess法测定一氧化氮(NO)含量,ELISA法检测前列腺素E_2(PGE_2)、白介素-6(IL-6)、肿瘤坏死因子(TNF)-α的含量,Western Blotting法检测诱导型一氧化氮合酶(iNOS)、环氧合酶(COX)-2等的表达。结果表明,青天葵黄酮F能显著降低LPS诱导的RAW264.7细胞中NO、PGE_2、IL-6和TNF-α的含量,抑制iNOS和COX-2蛋白的表达(P<0.05或P<0.01)。提示该化合物可能具有一定抗炎作用。
After treated with dexamethasone （positive control） or nervilifordin F extracted from Nerviliafordii （Hance） Schltr, RAW264.7 macrophage cells were stimulated by lipopolysaccharide （LPS） for 2 h. Nitric oxide （NO） production was analyzed by Griess reaction. The secretions of prostaglandin E2 （PGE2）, interleukin-6 （IL-6） and tumor necrosis factor （TNF） -α were analyzed by ELISA. The protein expressions of inducible nitric oxide synthase （iNOS） and cyclooxygenase （COX） -2 were analyzed by Western Blotting method. The results showed that nervilifordin F could inhibit the release of NO, PGE2, IL-6 and TNF-α, as well as the protein expressions of iNOS and COX-2 in RAW264.7 cells induced by LPS （P〈0.05 or P〈0.01）. It indicated that nervilifordin F might have anti-inflammatory effect.
出处
《中国医药工业杂志》
CAS
CSCD
北大核心
2014年第2期143-146,共4页
Chinese Journal of Pharmaceuticals
基金
国家自然科学基金资助项目(81260634)
广西自然科学基金(2013GXNSFAA019204
2013GXNSFBB053001)
关键词
青天葵黄酮F
毛唇芋兰
RAW264.7细胞
脂多糖
抗炎
nervilifordin F
Nerviliafordii （Hance） Schltr
RAW264.7 cell
lipopolysaccharide
anti-inflammatory
