核仁组成区嗜银蛋白染色在非霍奇金淋巴瘤骨髓涂片中的价值探讨

Investigate the value of argyrophilic nucleolar organizer region proteins on bone marrow smears of non-hodgkin's lym-phoma

目的探讨核仁组成区嗜银蛋白染色在非霍奇金淋巴瘤骨髓涂片中的价值。方法用经钟氏等报道[1]的双层滤纸垫染法对117例NHL骨髓涂片进行染色后检测,并另做17例大致正常骨髓片做对照。结果NHL组、NHL晚期组二者的银染颗粒含量和银染颗粒阳性率均较正常对照组有显著性差异(两组P<0.05);NHL晚期组的银染颗粒含量和银染颗粒阳性率均较NHL早期组有显著性差异(两者P<0.05);而NHL早期组的银染颗粒含量和银染颗粒阳性率均与正常对照组无显著性差异(P均>0.05)。结论AgNOR染色在NHL骨髓涂片的诊断中具有较大的应用价值。

Objective To explore the value of argyrophilic nucleolar organizer region proteins （AgNOR）on bone marrow smears of non-hodgkin＇s lymphoma （NHL）. Methods 117 cases of NHL bone marrow smears after double filter paper pad to stained by Zhong＇s et al reported wete detected,And another 17 cases normal bone marrow wete detected for comparison. Results There were significant difference both silver dye particles content and its stain positive rate in the NHL group together the early NHL phase group compared with normal group’s （two groups P both〈0.05）;The late NHL group silver staining granules and silver stain-ing positive rate of particles have significant difference compared with early NHL group （both P〈0.05）;But there were no signifi-cant difference in silver stained granules and it’s positive rate of early NHL group compared with normal group （P both〉0.05）. Conclusion Bone marrow slide AgNOR stain have great application value on NHL diagnosis.