基于SSR标记的水稻千粒重遗传剖析

Genetic Analysis of 1000-grain Weight in Rice Using SSR Marking Technique

为揭示控制水稻千粒重数量性状基因座(QTL)的动态表达,以亲本Milyang 46/FJCD建立的包含130个家系的F10重组自交系为材料,分析福建省武夷山和莆田环境下水稻灌浆期五个阶段千粒重的性状,并结合已构建的遗传图谱进行QTL动态定位及环境互作研究。结果表明:QTL分析共检测到22个加性QTL,位于1、2、3、4、5、6、7号染色体上,对表型变异贡献率为1.90%~24.78%。其中,9个QTL在不同的灌浆时期重复出现。环境互作分析共检测到8个QTL,位于1、2、3、4、5、6号染色体上,对表型变异贡献率为0~9.38%。在灌浆期第二、三、四、五阶段均检测到显著的基因与环境的互作效应,其中第五阶段检测到4个,第三阶段2个。

In order to reveal dynamical expression of QTL controlling 1000-grain weight, and the aim of this study contributed to high yield breeding in rice. An analysis of quantitative trait loci（QTL） and gene）〈 environment interaction（GE） were used to research 1000-grain weight for five stages of grain filling from a population of 130 F10 lines （recombinant inbred lines, RILs） deriving from Milyang 46 and FJCD. This research was carried out in Wuyishan and Putian, Fujian Province. Results. Twenty-two additive QTLs were detected in two environments by QTL mapping analysis, located in chromosome 1,2,3,4,5,6, 7 and the contribution rates for phenotype variance were from 1.9o//00 to 24.78~/0. Among them, nine QTLs expressed in different stages. Eight QTLs exited additive X environment interaction effect, located in chromosome 1, 2, 3, 4, 5, 6, and the contribution rates for phenotype variance were from 0 to 9.38~. QTI.s with additiveX environment interaction effect were detected in 2, 3, 4, 5 stage of grain filling, and 4, 2 QTLs exited in 5, 3 sta^e, respectively.