煤矸石地种植草木樨接种从枝菌根真菌的效应

Effects of AM Fungus on Infection and Growth of Melilotus albus Seedlings in Coal Gangue of Guiyang

为筛选出适宜在煤矸石废弃地生物修复所需的丛枝真菌,以煤矸石粉和耕作土壤作供试基质,进行了草木樨接种不同来源从枝菌根真菌(Arbuscular mycorrhizal fungus,AM)的培养试验,研究AM对草木樨侵染率、促生效应和草木樨对AM的依赖度。结果表明:摩西球囊霉(Glomus mosseae,Gm.)和根内球囊霉(Glomus intraradice,Gi.)菌株均能侵染草木樨根部,耕作土壤上的侵染率显著高于煤矸石粉;不同来源菌株的促生效应有差异,且地上部大于根系;草木樨对不同来源菌株的依赖度也有差异,初步认为草木樨与Gm.-GU(从贵州大学生命科学学院引进的摩西球囊霉)能形成优势共生体。在耕作土壤、煤矸石粉基质上接种Gm.-GU,菌根侵染率分别为69.7%和21.7%,地上部生物量、根系量、全株生物量和菌根依赖度均高于其他菌株。

To sift out a few arbuscular mycorrhizal fungus suitable for bioremediation in coal gangue, culture experiments were conducted by inoculating with AM fungus in coal gangue and upland soil to study the effects of AM fungus on infection and growth of M. albus seedlings. The results showed that G. mosseae and G. intraradice could all infect the root of M. albus seedlings. The infection rate was significantly higher in cultivated soil than in coal gangue; The difference of improving the M. albus growth by inoculating with different AM fungus strains was observed, which was bigger in overground part than in the root; The dependence of M. albus to AM fungus was obviously observed. M. albus and Gm.-GU could form dominant symbiont. The infection rates was respectively 69.7% and 21.7% in cultivated soil and coal gangue matrix. The dependence of M. albus to AM fungus of overground biomass, root quantity and complete stool biomass was higher than other strains.