白藜芦醇对认知损伤老年小鼠海马凋亡相关蛋白的影响

The effects of resveratrol on the apoptosis-related proteins in the hippocampus in the isoflurane-induced cognition impairment aged mice

目的观察白藜芦醇对异氟醚吸入诱导的认知损伤老年小鼠海马凋亡相关蛋白表达的影响。方法 15月龄雄性C57BL/6小鼠24只随机分为4组(n=6):对照组(A组)、异氟醚组(B组)、白藜芦醇+异氟醚组(C组)、白藜芦醇组(D组)。A和B组分别于连续7 d腹腔注射生理盐水后吸入氧气和1.5%异氟醚;C和D组分别于连续7 d腹腔注射白芦藜醇后吸入1.5%异氟醚和氧气。第8天进行条件恐惧性实验,行为学测试后取小鼠海马组织,检测caspase-3、Bax和Bcl蛋白水平。结果与A组相比,B组环境诱发僵直时间百分比下降(P<0.05);与B组相比,C组环境诱发僵直时间百分比升高(P<0.05);各组间声音诱发僵直时间百分比差异无统计学意义。与A组相比,B组小鼠海马中的caspase-3和Bax水平升高(P<0.05),Bcl水平下降(P<0.05);与B组相比,C组小鼠海马中的caspase-3和Bax水平下降(P<0.05),Bcl水平升高(P<0.05)。结论白藜芦醇可能通过调节老年小鼠海马凋亡相关蛋白的表达,改善异氟醚吸入诱导的认知功能损伤。

Objective To study the effects of resveratrol on the apoptosis-related proteins in the hippocampus in the isoflurane-induced cognition impairment aged mice. Methods Twenty-four fifteen-month male C57BL/6 mice were equally randomized into 4 groups（n=6）： control group（A group）, isoflurane group（B group）, resveratrol＋isoflurane group（C group） and resveratrol group（D group）. The mice in the A and B group received O2 and 1.5%isoflurane exposure for 2 h, respectively, after the intraperitoneal injection of saline daily for 7 consecutive days. The mice in the C and D group received 1.5%isoflurane and O2 exposure for 2 h, respectively, after the intraperitoneal injection of resveratrol daily for 7 consecutive days. All the mice were subjected to the fear conditioning test on the eighth day. The western blot was applied to detect the hippocampal levels of caspase-3, Bax, and Bcl after the fear conditioning test. Results Compared with A group, the percentage of the contextual conditional freezing time decreased significantly in B group（P〈0.05）. Compared with B group, the percentage of the contextual conditional freezing time increased significantly in C group（P〈0.05）. There was no significant difference in the percentage of the tone conditional freezing time among the groups （P〉0.05）. Compared with A group, the hippocampal caspase-3 and Bax increased significantly and the hippocampal Bcl decreased significantly after the isoflurane anesthesia in B group（P〈0.05）. Compared with B group, the hippocampal caspase-3 and Bax levels decreased significantly and the hippocampal Bcl increased significantly after the isoflurane anesthesia in C group （P〈0.05）. Conclusion Resveratrol may attenuate the cognitive impairment induced by isoflurane anesthesia in aged mice by regulating the apoptosis-related proteins in the hippocampus.