甜地丁中槲皮素的薄层鉴别和含量测定

Identification of Quercetin in Herba Gueldenstaedtiae by TLC and Determination

为完善甜地丁质量标准，采用薄层色谱法对甜地丁中槲皮素的鉴别进行了研究，并用高效液相色谱法测定其含量。薄层色谱以甲苯-乙酸乙酯-甲酸（7：5：0．5）为展开剂，高效液相法色谱柱为C18（4．6mm×250mm，5μm），流动相为甲醇-0．3％磷酸溶液（50：50），检测波长为360nm，流速1mL／min，柱温25℃，进样量10μL。结果表明薄层色谱法能对槲皮素实现有效分离，高效液相色谱法槲皮素进样浓度在5。100μg／mL范围内与峰面积呈良好的线性关系（r=O．9997）；样品平均回收率为96．1％（n=6），RSD为0．7％。本方法为进一步控制甜地丁的质量提供了依据。

In order to improve the quality standard of Herba Gueldenstaedtiae , an TLC method for identification of quercetin in Herba Gueldenstaedtiae was developed. And it＇ s content was determination by HPLC. The TLC developing agent was methylbenzene - ehhlacetate - methanoic acid（7 ： 5 ： 0.5）.The HPLC conditions was use C18 column（4.6 min×250 ram,5 μm） ,mobile phase was methanol - 0.3% phosphoric acid solution （50 ： 50）, detection wavelength was 360 nm, flow rate was 1 mL/min , column temperature was 25 ℃, the injection volume was 10 p.L. Quercetin was effectively speparated by TLC, and there was a good linear correlation between the peak areas and the concentrations of quercetin at the range of 5- 100 μg/mL（r=0.9997）by HPLC.The average recovevery was 96.1% with RSD of 0.7%（n=6）. It can be used for the quality control of quercetin in Herba Gueldenstaedtiae.