摘要
As an important intermediate substance in the process of producing 1,3-propanediol （1, 3-PD） by bio-dissimilation of glycerol, the concentration of 3-hydroxypropionaldehyde （3-HPA） has inhibitory action on glycerol dehydratase （GDHt） and 1, 3-propanediol oxi- doreductase （PDOR）. Considering the transmission of glycerol and 1,3-PD across cell membrane by both passive diffusion and active transmission as well as the inhibitory action of 3-HPA on the specific rate of growth, this paper aims to establish and dis- cuss a nonlinear hybrid dynamical system model to ascertain the concentration range of 3-HPA where it does exhibit an inhibitory action on GDHt and PDOR. A quantita- tive definition of biological robustness is presented and an identification model is estab- lished based on biological robustness. An algorithm procedure is constructed to solve the identification problem. Numerical results show that only within a proper concentration range 3-HPA can have inhibitory action on the two enzymes mentioned above.
