评价抗SSA、抗SSB和抗α-胞衬蛋白抗体在诊断干燥综合征中的价值

Diagnostic Value of Anti-SSA,Anti-SSB and Anti-alpha-fodrin Antibody in Sjogren's Syndrome

目的:评价SSA、SSB和胞衬蛋白在诊断干燥综合征(SS)中的价值。方法:用酶联免疫吸附试验(ELISA)检测47例确诊为SS患者、20例其它自身免疫病(类风湿关节炎7例、SLE6例、自身免疫性肌炎7例)和20例体检健康者血清抗SSA、SSB和α-胞衬蛋白抗体,分析三种自身抗体阳性率。结果:抗SSA抗体、抗SSB抗体和抗α-胞衬蛋白抗体敏感性分别为76.6%、38.3%、42.6%;特异性分别为72.5%、95.0%、82.5%;三种抗体联合检测后敏感性为89.4%,特异性50.0%。结论:抗α-胞衬蛋白抗体与抗SSA、SSB抗体联合检测时可提高阳性率,对症状不典型的SS患者诊断有一定的补充意义。

Objective： To assess the diagnostic value of anti-SSA, anti-SSB and anti-alpha-fodrin antibody in Sjogren＇s Syndrome （SS）. Methods： Serum samples were collected from 47 patients with SS, 20 patients with other autoimmune diseases（including 7 cases of rheumatoid arthritis, 13 cases of systemic lupus erythematosus）and 20 normal human controls. Enzyme-linked immunosorbent assay （ELISA） and immunoblotting were performed to test anti-SSA, anti-SSB and anti-alpha-fodrin antibody in these serum samples. Results： The sensitivities of anti-SSA, anti-SSB and anti-alpha-fodrin antibody in SS were 76.6%, 38.3%aesnnd 42.6%, respectively, and the speeificities were 72.5%, 95.0% and 82.5%, respectively. The combination of anti-SSA, anti-SSB and anti-alpha-fodrin antibody had a higher sensitivity（89.4%）, but it had a lower sensitivity（50.0%）. Conclusions：The combination of anti-SSA, anti-SSB and anti-alpha-fodrin antibody can significantly improve the sensitivity to diagnose SS and offer clinical benefits for diagnosis of SS whose symptoms are not typical.