TGF-β1和IGF-1 mRNA在早期胚胎停育绒毛组织中的表达及意义

Study on Expression of TGF-β1 and IGF-1 mRNA in the Chorionic Villi of Early Embryo Growth Arrest

目的:通过检测胚胎停育患者绒毛组织中TGF-β1和IGF-1 mRNA的表达,探讨其在胚胎停育发病机制中的作用。方法:用半定量逆转录聚合酶链反应(RT-PCR)技术检测正常人流(20例),胚胎停育(25例)绒毛组织中TGF-β1和IGF-1基因在转录水平的表达。结果:(1)与对照组相比,实验组绒毛组织中TGF-β1mRNA表达量降低(P<0.05),(2)胚胎停育患者绒毛中IGF-1mRNA表达量升高(P<0.05)。(3)绒毛组织中TGF-β1与IGF-1的表达呈负相关(r=-0.793,P<0.05)。结论:TGF-β1、IGF-1的表达在基因转录水平发生改变,TGF-β1表达的降低可能上调了IGF-1的表达,提示两者可能共同参与了胚胎停育的发生。

Objective： Onto investigate the expression of TGF-β1 and IGF-1 mRNA in the ehorionic villi of early embryo growth arrest in order to evaluate the role of early embryo growth arrest. Methods： Reverse transcription polymerase chain reaction （RT-RCR） was used in the normal groups （20 cases ） and the early embryo growth arrest group （25 cases ） to quantitatively determining TGF-131 mRNA and IGF-I mRNA in the ehorionie villi tissues. Their quantities were noted by the density of TGF-β1/[3-aetin or IGF-1/β-actin. Results： （1） Compared with that in the normal group, the mRNA expression level of TGF-β1 of early embryo growth arrest was lower （ P 〈0.05）, （2） The levels of IGF-1 was significantly higher （P 〈0.05）. （3） There was a negative correlation between the TGF-β 1 and IGF-1 in ehorionic villi tissues（ r=-0.793, P〈0.05）. Conclusion： The abnormal expression of the TGF-β1 and IGF-1 was already changed at the level of transcription. TGF-β1 may increase the expression ofIGF-1 in some extent, which indicated that they might be involved in the pathogesis of early embryo growth arrest.