肺炎衣原体感染与青少年I型糖尿病的相关性研究

Correlational Study on the Chlamydia Pneumonia Infection with Adolescent Type1 Diabetes Mellitus

目的:探讨肺炎衣原体感染与青少年I型糖尿病的相关性,为I型糖尿病的的临床治疗提供参考依据。方法:选择2010年12月-2012年6月间石家庄地区各医院收治的49例青少年T1DM患者为观察组,及同期50例健康人作为对照组,应用即时指尖血免疫测定仪分析受试者HbA1c水平;应用RT-PCR技术检测血液中Cpn DNA;应用ELISA方法检测受试者血清中Cpn特异性抗体水平,对Cpn DNA的检出情况及HbA1c水平与Cpn DNA和特异性抗体水平的相关性进行统计学分析。结果:观察组Cpn DNA的检出率为46.9%,显著高于对照组(P<0.05);观察组Cpn抗体阳性率显著高于对照组(P<0.05),且观察组再次感染或慢性感染Cpn的百分率显著高于对照组(P<0.05);HbA1c与IgG/IgA抗体水平显著相关,血糖控制较差(HbA1c>9%)的糖尿病患者Cpn IgG/IgA抗体阳性率与血糖控制较好的患者(HbA1c<7%)相比显著升高(P<0.05)。结论:与健康对照相比,青少年T1DM患者更容易感染Cpn,且更容易由急性感染状态进展为慢性感染形式,良好的血糖可能降低患者发生与代谢控制有关的慢性并发症。

Objective： To evaluate the association between Chlamydia pneumoniae（Cpn） infection and adolescent Typel Diabetes Mellitus （T1DM）, and provide basis for the clinical treatment of T1DM. Methods： HbAlc was measured by the point-of-care immunoassay; Cpn DNA was detected by real time PCR; The serum Cpn specific antibody IgA, IgG, IgM were measured by ELISA. Results： Cpn DNA was performed in 46.9% of the patients with T1DM, this prevalence was higher than that in control group（P〈0.05）; Cpn antibody and re-infection or chronic infection rate were higher in observation group than those in the control group （P〈0.05）; There was a significant positive correlation between the HbAlc and the IgG/IgA. Cpn IgG/IgA antibody positivity was significantly （P〈0.05） more common in HbAlc〉9% patients versus HbAlc〈7% patients. Conclusion： Adolescents with T1DM were more likely to show signs of infection with Cpn, and have an increased risk from an acute Cpn infection to a chronic form. Good glycaemie control may reduce the chronic complications related to metabolic control.