萱草优良单株花茎离体快繁

Rapid Propagation in vitro of Hemerocallis Pedicels in the Superior Individuals

以4个萱草材料优良单株的花茎为试材,接种于不同激素组合的培养基中,对花茎不同部位的启动、不定芽分化及增殖进行研究。结果表明:最佳外植体为花茎上部和中部,其中花茎上部在启动培养中只呈现出间接不定芽发生途径;而中部呈现出间接和直接不定芽发生两种途径。4个萱草材料在启动培养、增殖培养中呈现出差异性,其中CC627较其它3个材料呈现出较强的优势,启动率最高为62.53%、增殖率可高达100%。WNF和L-1最适启动培养基为MS+6-BA1.0 mg/L+NAA0.5 mg/L+2,4-D1.0 mg/L;RBH最适启动培养基为MS+6-BA3.0 mg/L+NAA1.0 mg/L;CC627最适启动培养基为MS+6-BA2.0 mg/L+NAA0.5 mg/L+2,4-D1.0 mg/L。WNF和L-1最适增殖培养基为MS+6-BA2.0 mg/L+NAA0.2 mg/L;RBH最适增殖培养基为MS+6-BA3.0 mg/L+NAA0.2 mg/L;CC627最适增殖培养基为MS+6-BA1.0 mg/L+KT1.0 mg/L+NAA0.2 mg/L。

The experiment was conducted to inoculate four kinds of Hemerocallis materials ’ superior individual pedicellexplants into mediums for different hormone combination to study initiation from different parts of the pedicels, differentiation and proliferation of adventitious bud.The best explant is the pedicellof upside and middle part, the initiation way is indirect adventitious bud for upside pedicell, while the initiation way is both indirect and direct adventitious bud for middle pedicell. Four kinds of Hemerocallis present otherness during initiation and proliferation culture.Compared with other three kinds of materials, CC627 presents a stronger advantage, the maximum of initiation rate is 62.53%, and the proliferation rate can get to 100%.The optimum initiation medium of WNF and L-1 is MS＋6-BA 1.0 mg/L＋NAA 0.5 mg/L＋2,4-D 1.0 mg/L, the optimum initiation medium of RBH is MS＋6-BA 3.0 mg/L＋NAA 1.0 mg/L, and the optimum initiation medium of CC627is MS＋6-BA 2.0 mg/L＋NAA 0.5 mg/L＋2,4-D 1.0 mg/L.The optimum proliferation medium of WNF and L-1 is MS＋6-BA 2.0 mg/L＋NAA 0.2 mg/L. The optimum proliferation medium of RBH is MS＋6-BA 3.0 mg/L＋NAA 0.2 mg/L.The optimum proliferation medium of CC627 is MS＋6-BA 1.0 mg/L＋KT 1.0 mg/L＋NAA 0.2 mg/L.