壳聚糖双胍盐酸盐抑菌活性及其涂膜保鲜圣女果的研究

Effects of chitosan biguandine hydrochloride coating on storage quality of cherry tomato and its antimicrobial activities

测定了壳聚糖双胍盐酸盐(CGH)对金黄色葡萄球菌、大肠杆菌和番茄丝核菌的抑菌能力;并通过涂膜的方法处理圣女果,测定贮藏过程中果实失重率、硬度、可溶性固形物含量(TSS)、维生素C(VC)含量、细胞膜透性和总酸含量的变化。结果表明,相对于壳聚糖(CTS),CGH对金黄色葡萄球菌、大肠杆菌具有较强的抑菌能力,其最小抑菌浓度(MIC)分别为0.31mg/mL和0.62mg/mL;当CGH浓度达到2.00mg/mL时对番茄丝核菌菌丝生长抑制率可达到100%。以质量浓度为3%的CGH涂膜处理的圣女果在(28±2)℃存放12d后,失重率仅为7.5%,硬度仍有21.121N/cm2,TSS为7.2%,VC含量为21.70mg/100g FW(鲜重),膜渗透率为27.4%,可滴定酸含量为0.35%。与对照(CK)相比,显著保持了果实的品质。

The antimicrobial activities of Chitosan biguandine hydrochloride（CGH） against S. Aureus,E. Coli and Rhizoctonia solani Kuhn were studied, respectively. Cherry tomatoes mass -loss ratio, firmness, total soluble solid, V c content, film penetration ratio and soluble acid content was examined attentively after fresh -keeping process with CGH as coating film. The minimal inhibited concentrations （MIC） against S. Aureus and E. Coli were 0.31 and 0.62mg/mL,respectively. CGH at 2.00mg/mL had a total antifungal effect on Rhizoctonia solani Kuhn. CGH with concentration of 3% coated on the cherry tomatoes showed a better preservation effect after 12d storage under （28±2）℃. The mass-loss, firmness, total soluble solid, Vc content, film penetration ratio and soluble acid content were 7.5%, 21.121N/cm2 ,7.2%,21. 70mg/100g FW （fresh weight）, 27.4% and 0.35%, respectively.