基于门控制效应的氯霉素分子印迹传感器研制

Determination of Chloramphenicol by Gate Control of Molecularly Imprinted Polymer Sensor

研究了分子印迹技术与电化学检测手段相结合的分子印迹电化学传感器，并用于定量分析蜂蜜中的氯霉素含量。以亚甲基蓝为功能单体、氯霉素为模板分子，在pH＝7.0的磷酸盐缓冲溶液中采用循环伏安法在玻碳电极表面电聚合形成分子印迹敏感膜，并以此作为识别元件制备了电流型氯霉素分子印迹电化学传感器。采用铁氰化钾作为探针分子，利用门控制效应对氯霉素进行定量检测。氯霉素浓度在0.323～54.9μg/L范围内与峰电流呈良好的线性关系。检出限（3δ）为0.14μg/L。该传感器具有灵敏度高、选择性好、成本低且容易制备的优势。

In this paper, a sensitive sensor based on the molecularly imprinted polymer （ MIP） of methylene blue （ MB） is proposed, and its configuration and performance are studied .The progress of electro-polymeriza-tion is performed in a phosphate buffer solution （ pH=7.0 ） containing 1.0 mmol/L chloramphenicol and 3.0 mmol/L MB on the surface of a glassy carbon electrode.The trait of molecularly imprinted polymer membrane is characterized by cyclic voltammetry.The molecularly imprinted electrochemical sensor is conducted by the gate control of MIT and potassium ferricyanide act as probe molecules . The amount of chloramphenicol mole-cules is measured by differential pulse voltammetry （ DPV） .The linear range is 0.323~54.9 μg/L.The de-tection limit is 0.14 μg/L.