西南轮环藤的化学成分及箭毒碱型生物碱的结构研究

Study on the chemical constituents of Cyclea wattii and structure of curines- type alkaloids

目的研究西南轮环藤CycleawattiiDiels根中的化学成分，确定箭毒碱型异喹啉生物碱的绝对构型。方法利用色谱分离化合物单体，通过CD、MS、NMR、X—ray等技术鉴定化合物的结构。结果分离得到7个单体成分，分别为7—O-meth—ylhayatidine（I）、racemosidineB（II）、racemosidineC（m）、racemosimineA（1V）、胡萝卜苷（V）、β-谷甾醇（VI）和棕榈酸甘油酯（Ⅶ）。验证了前期分离得到的左旋箭毒碱（-）-curine的立体结构；总结了箭毒碱型生物碱的CD谱规律。结论化合物I～Ⅶ均为首次从该植物中分离得到；1-S、1’-R型与1-R、1＇-R型箭毒碱型生物碱的CD谱差异明显。

OBJECTIVE To investigate the chemical constituents of Cyclea wattii, and elucidate structures of curines - type bis- benzylisoquinoline alkaloids. METHODS The compounds were isolated by chromatography, and their structrues were identified by CD, MS, NMR and X - ray analyses. RESULTS Seven compounds were isolated from the ethanol extract of the roots of Cyclea wat- tii. Their structrues were identified as 7 - 0 - methylhayatidine （ I ） , racemosidine B （ l] ） , racemosidine C （ Ill ） , racemosimine A （ IV ） ,daucosterol（ V ）, [3 - sitosterol（ VI ） and a - palmitoylglycerol（ VII ）. Absolute conFigureurations of （ - ） - curine separated in the preceding paper was confirmed by X - ray crystallographic analysis, and the CD spectrum of curines - type alkaloids was carried out. CONCLUSION Compounds I - VII were isolated from this plant for the first time.