丹参酮ⅡA脂质体的制备及对HepG2/ADR细胞增殖的抑制作用

Preparation of tanshinone ⅡA liposomes and its growth inhibitory effects on HepG2/ADR cells

目的研制丹参酮ⅡA(TanⅡA)脂质体,考察其对人肝癌HepG2/ADR阿霉素耐药细胞增殖的抑制作用。方法采用薄膜分散法制备TanⅡA脂质体,改良MTT法测定药物肿瘤细胞增殖的抑制作用。结果制备载药脂质体的平均粒径为68nm,Zeta电位为-23.4 mV;载药量与包封率分别为138.9±8.7μg·mL-1、97.4%±1.2%(n=3);与游离药物相比,TanⅡA脂质体对HepG2/ADR的增殖细胞有更强的抑制(P<0.05),并呈时间和剂量依赖性。结论 TanⅡA脂质体具有治疗多药耐药肝癌的前景。

OBJECTIVE To develop liposomes （LP） of tanshinone II A （ Tan II A） and investigate growth inhibitory effects of the Tan HA liposomes （ Tan IIA - LP） on HepG2/ADR cells. METHODS The Tan IIA - LP was prepared by film dispersion method. The inhibitory effects of Tan I]A - LP and free Tan HA on the HepG2/ADR ceils were evaluated by modified MTT assay. RESULTS The mean particle size of prepared Tan IlA - LP was 68 nm with Zeta potential of - 23.4 inV. The drug - loading efficiency and entrapment efficiency of Tan IIA - LP were 138.9 ±8.7 μg·. mL-1 and 97.4% ± 1.2% （ n = 3 ） , respectively. Compared with free Tan IIA, Tan IIA - LP was significantly more effective than the free Tan IIA in HepG2/ADR cells. CONCLUSION The results suggest that the Tan IlA - LP would be a promising drug delivery system on treatment of drug - resistant hepatocelluar carcinoma.