摘要
建立了冷饮中7种人工合成色素的高效液相色谱分析方法。样品用聚酰胺小柱吸附法提取色素，以甲醇-乙酸铵溶液为流动相梯度洗脱，通过C18反相色谱柱分离，紫外检测器245nm检测。结果表明柠檬黄、苋菜红、靛蓝、胭脂红、日落黄和诱惑红在0.3～100mg/kg，亮蓝在1～100mg/kg范围内呈良好的线性关系。7种色素的回收率为75.3%～102.7%。本方法用于冷饮中7种合成色素的测定，分离效果好，定量准确，重复性好。
A method was established for the determination of 7 synthetic pigments in cold drinks by high performance liq-uid chromatography （HPLC）. Synthetic pigments in samples were extracted by a polyamide column and then separated on a C18 column with methanol and ammonium acetate as the mobile phase,and detected with a UV detector at 245nm. The re-sults showed that tartrazine,amaranth,indigo,carmine,sunset yellow and fancy red had a good linear relationship in the range of 0.3-100mg/kg,and that brilliant blue had the same situation in the range of 1-100mg/kg. The recovery rate of the 7 pigments was 75.3%-102.7%. The method showed good separation results and high accuracy and reproducibility when used for the determination of 7 synthetic pigments in cold drinks.
出处
《饮料工业》
2014年第1期27-29,共3页
Beverage Industry
