七叶皂苷钠对重症急性胰腺炎模型大鼠的作用探讨

Therapeutic effect of sodium aescinate on severe acute pancreatitis in rats

目的 研究七叶皂苷钠对重症急性胰腺炎（SAP）大鼠的治疗作用.方法 采用胆胰管逆行注射5％牛磺胆酸钠的方法制备SD大鼠SAP模型,按随机数字表法随机分为假手术组、SAP模型组、七叶皂苷钠组各16只.七叶皂苷钠组尾静脉注射七叶皂苷钠5 mg/kg、给药体积为10 ml/kg,假手术组和SAP模型组尾静脉注射等体积生理盐水.术后6h、12h采用比色法检测血清淀粉酶,光镜下观察各组胰腺组织的病理学改变.结果 术后6h、12h SAP模型组大鼠胰腺组织出现损伤,其病理评分分别为（9.42±1.06）分、（18.30±2.18）分,与假手术组比较[术后6h、12 h分别为（0.55±0.20）分、0分]差异有统计学意义（P＜0.01）.七叶皂苷钠组胰腺组织术后6h、12h病理评分分别为（7.85±1.33）分、（12.75±1.69）分,炎性渗出和炎症反应较SAP模型组明显减轻.术后6h、12h SAP模型组大鼠血清淀粉酶升高[术后6h、12h分别为（2612±59） U/L、（3004±687） U/L],与假手术组[术后6h、12h分别为（928±271） U/L、（890±295） U/L]比较差异有统计学意义（P＜0.01）.七叶皂苷钠组术后6h、12h的血清淀粉酶水平分别为（1790±336） U/L、（2093±298） U/L,均较SAP模型组术后6h、12h显著降低（P＜0.01）.结论 七叶皂苷钠对SAP模型大鼠的胰腺组织具有保护作用.

Objective To investigate the therapeutic effect of sodium aescinate （SA） on severe acute pancreatitis （SAP） in rats.Methods SAP model was induced by intraductal administration of 5％ taurocholic acid sodium salt.SD rats were then randomly divided into 3 groups （n =16）,namely sham,model and SA group （5 mg/kg）.Drugs or saline（10 ml/kg） were administered via caudal vein 30 min post-operation.The serum amylase was measured with spectrophotometer and pancreatic histological changes were observed under microscope 6 h and 12 h post-operation,respectiveiy.Results Pancreatic histological grades in the model group were （9.42 ± 1.06） and （18.30± 2.18） 6 h and 12 h post-operation,respectively,which showed significant differences （P＜0.01） compared with the sham group [（0.55 ±0.20） and 0,respectively].SA evidently reduced the severity of pancreatic pathology and improved the tissue inflammation in rats with SAP.Pancreatic histological grades were （7.85±1.33） and （12.75±1.69）in the SA group 6 h and 12 h post-operation,respectively,which had significant differences compared with the model group （P＜0.05）.Serum amylase levels in model group were （2612 ± 59） U/L and（3004±687）U/L6 h and 12 h post-operation,respectively,which showed significant differences （P ＜ 0.01） compared with the sham group [（928 ± 271） and （890± 295） U/L,respectively].Serum amylase levels were reduced to （1790± 336） U/L and （2093 ± 298） U/L in the SA group 6 h and 12 h post-operation,respectively.SA markedly inhibited the level of serum amylase compared to the model group （P＜0.01）.Conclusion SA could protect rats with SAP effectively.