补肾益心片对高血压模型大鼠一氧化氮合酶、一氧化氮及血管紧张素1-7的干预作用

Intervention effect of Bushen-Yixin tablet on the level of NOS, NO and Ang （1-7） in spontaneously hypertensive rat

目的 观察补肾益心片对高血压大鼠（SHR）一氧化氮合酶（NOS）、一氧化氮（NO）及血管紧张素1-7 （Ang 1-7）的调节作用.方法 将原发高血压雄性大鼠按随机数字表法随机分为补肾益心片组（8只）、缬沙坦组（8只）、高血压模型组（7只）,分别予以补肾益心片、缬沙坦、生理盐水灌胃.治疗4周后,检测大鼠血浆、腹主动脉组织内NOS、NO浓度以及血浆Ang1-7浓度.结果 补肾益心片组可降低血浆NO（15.500±4.375）μmol/L、提高腹主动脉NO （35.604±7.505） μmol/L及血浆Ang1-7（45.542±12.726） ng/L,与高血压模型组[分别为（26.000±2.828）μmol/L、（17.437±11.649） μmol/L、（25.521±2.727） ng/L].比较,差异有统计学意义（P＜0.05）.其中,补肾益心片组大鼠腹主动脉NO水平较缬沙坦组[（21.537±13.484） μmol/L]显著升高,差异有统计学意义（P＜0.05）.结论 补肾益心片可增加高血压大鼠腹主动脉NO、血浆Ang1-7含量.

Objective To study the variation of the level of NOS,NO and Ang （1-7） in spontaneously hypertensive rat（SHR）.Methods SHR were divided randomly into 3 groups：Bushenyixin group（n=8）,valsartan group （n=8）,and model group （n=7）.Each group was given Bushenyixin tablet,valsartan,and saline by gavage respectively.After 4 weeks,the concentration of NOS,NO,Ang （1-7） in plasma and the concentration of NOS,NO in aorta were be observed.Results The concentration of NO in plasma was significantly lower in the Bushen-Yxin group than for model group（15.500±4.375）μmol/L vs.（26.000±2.828）gmol/L,P＜0.05.The concentration of NO in aorta and Ang1-7 in plasma were significantly higher in the Bushen-Yixin group than in the model group [（35.604±7.505）μmol/L vs.（17.437± 11.649） μ mol/L,（45.542 ± 12.726）ng/L vs.（25.521 ± 2.727） ng/L,P＜0.05].The concentration of NO in aorta was significantly higher in Bushen-Yixin group than in the valsartan group [（21.537± 13.484） μmol/L,P＜0.05].Conclusion Bushen-Yixin tablet had effects of increasing of NO level in aorta and Ang （1-7） level in plasma in spontaneously hypertensive rat.