金黄色葡萄球菌抗生素耐药相关基因检测

Detection of antibiotic-resistant genes of Staphylococcus aureus

目的检测医院及环境样中金黄色葡萄球菌(Staphylococcus aureus,SA)耐药基因,了解耐甲氧西林的金黄色葡萄球菌(Methicillin Resistant Staphylococcus aureus,MRSA)和甲氧西林耐药凝固酶阴性葡萄球菌(Methicillin Resistant coagulase Staphylococcus aureus,MRCNS)检出率以及金黄色葡萄球菌耐药基因分布情况。方法应用多重聚合酶链反应(Multiplex polymeras chain reaction,multiplex PCR)检测医院及环境样中金黄色葡萄球菌的mecA、femA基因。采用聚合酶链反应(Polymeras chain reaction,PCR)技术检测金黄色葡萄球菌的11种耐药基因,分别是β-内酰胺类耐药基因blaTEM,氨基糖苷类耐药基因Aac(6')/aph(2'')、aph(3')-Ⅲ、ant(3'')-Ⅰ、ant(4',4'')与ant(6)-Ⅰ,四环素耐药基因tetM,红霉素耐药基因erm,万古霉素耐药基因vanA、vanB,耐消毒基因qacA。结果 44株菌分离自医院病人样本,检出12株MRSA,3株MRCNS。其中29株菌携带1种以上耐药基因,14株携带tetM基因,5株携带vanB基因,7株携带Aac(6')/aph(2'')基因,9株携带aph(3')-Ⅲ基因,7株携带ant(4',4'')基因,5株携带ant(6)-Ⅰ基因。29株菌分离自环境样本,未检出MRSA与MRCNS。仅1株检出Aac(6')/aph(2'')基因,16株检出ant(4',4'')基因,其余10种耐药基因未检出。结论院内样本中,不同样本分离菌株携带耐药基因存在差异,未检出携带qacA基因的菌株。环境样本携带ant(4',4'')基因较高。检测耐药基因分布情况,为指导临床用药、控制院内感染及监测环境中菌株耐药情况提供依据。

Objective To investigate the antibiotics gene distribution of Staphylococcus aureus isolated from the hospital and environment＇s samples as well as detection rate of MRSA and MRCNS in order to guide clinical treatment and infection control. Methods Multiplex polymerase chain reaction （PCR） was employed to detect the antibiotic genes mecA. femA of Staphylococcus aureus and 11 kinds of antibiotic-resistant genes blara,Aac （6＇）/aph（2＂ ）.aph（3＇ ）- ]U .ant（3＂ ）- I .ant（4＇,4＂） .ant（6）- I .tetM.erm.vanA.vanB.qacA. Results 12 strains MRSA and 3 strains MRCNS were detected in 44 strains of staphylococcus aureus from samples of patients. 14 with term genes,5 with vanB genes,7 with Aac（6＇）/aph （2＂） genes,9 with aph（3＇）-III genes,7 with ant（4＇,4＂） genes,5 with ant（6）- I genes. The MRSA and MRCNS were not found in environment＇s samples. A ac （6＇）/aph（2＂） gene was detected in only one strain, ant（4＇ ,4＂） gene were detected in 16 strains,the other 9 kinds antibiotic-resistant genes were not detected. Conclusions There was some difference of antibiotic-resistant genes between patients and environment. All strains didn＇t carry the disinfectant-resistant gene of qacA. The ant（4＇,4＂） genes earring rate was higher in environment＇s samples.