金雀异黄素对LPS诱导白色念珠菌感染干预作用

Effect of genistein against C.albicansinfection with LPS activated macrophage

摘要：采用荧光标记（CFSE）法观察脂多糖（LPS）和O～100μmol·L^-1的金雀异黄素与巨噬细胞RAW264．7共刺激后细胞增殖的变化；再用荧光定量法检测不同刺激下巨噬细胞对白色念珠菌的吞噬作用，以探讨细菌内毒素LPS对巨噬细胞RAW264．7吞噬白色念珠菌的影响以及金雀异黄素的干预作用。结果表明：LPS单独刺激巨噬细胞20h后，对细胞增殖没有显著影响（P〉0．05），而33．3和100．0μmol·L^-1的金雀异黄素抑制巨噬细胞增殖，分别为对照组的65％和71％（P〈0．01）。LPS预刺激巨噬细胞24h后，在白色念珠菌感染15～45min内，巨噬细胞对白色念珠菌的吞噬作用显著增强，而浓度为3．7～11．1μmol·L^-1的金雀异黄素能显著拮抗这一作用（P〈0．01）。说明LPS能够显著诱导巨噬细胞对活白色念珠菌吞噬作用，金雀异黄素在细菌和白色念珠菌共存条件下对宿主有保护作用。

ABSTRACT： Objective： To investigate the influence of LPS on the macrophage against C. albicans infection and to explore the protective effect of genistein. Method： Cell proliferation after 20h co-culture with LPS and different concentration of genistein was detected by CFSE assay; Phagocytosis was determined by a fluorometric assay. Result： LPS alone had no effects on the cell proliferation of macrophage after 20 h stimulation （P〉0. 05）. Whereas, genistein（33. 3, 100. 0μmol·L^-1 ） showed significant inhibition on the cell proliferation, which was 65% and 71% of the control （P〈0. 01）. During 15-45 min infection periods, LPS strongly enhanced the phagocytosis of macrophage against viable C. albicans. And genistein could significantly inhibit this effect, the effective concentration range was 3.7-11.1μmol·L^-1 （p〈0.01）. Conclusion： LPS could induce the susceptibility of C. albicans to macrophage, and genistein could strongly protect macrophage from C. albicans infection during bacteria infection.