摘要
目的:研究Wnt5a对Wnt3a处理过的melan-a细胞分泌黑色素的影响。方法:体外培养黑色素细胞(melan-a细胞),分别进行GFP、Wnt3a、Wnt3a+Wnt5a处理,比较细胞的突起,酪氨酸酶的活性以及黑素合成相关基因(TYR、TRP2、MITF)的表达情况。结果:Wnt3a促进黑色素细胞突起的生长和TYR、TRP2、MITF的表达,而Wnt5a逆转了Wnt3a对黑色素细胞的作用。结论:Wnt5a抑制Wnt3a促黑素细胞黑素生成的作用,表明在melan-a黑素细胞中Wnt5a可有效抑制Wnt经典通路。
Objective： To investigate the effects of Wnt5a on melanogenesis in melanocytes treated by Wnt3a. Methods： Melanocytes （melan-a cells） were cultured in vitro. The cell protrusions and Tyrosinase activity treated with different adenovirus （AdGFP, AdWnt3a, AdWnt3a＋AdWnt5a） were determined, and the mRNA expression of MITF （Microphthalmia-associated transcrip- tion factor）, TRP2 （Tyrosinase-related protein 2） and Tyrosinase were also studied by reverse-transcription polymerase chain reaction （RT-PCR）. Results： Wnt3a promoted the growth of melan-a cells protrusions and the expression of TYR, TRP2, MITF. Wnt5a reversed the effect of Wnt3a. Conclusion： Wnt5a inhibits melanogenesis brought by Wnt3a in melanocytes, indicating that the suppression role of Wnt5a is playing in the noncanonical Wnt signaling pathway.
出处
《现代生物医学进展》
CAS
2013年第36期7013-7016,共4页
Progress in Modern Biomedicine
基金
国家自然科学基金项目(81101205)
本科生教学改革课题(2011B11)
