慢性乙型肝炎病毒DNA定量检测与慢性乙肝、肝脏损伤程度、HBeAg关系的探究

The Clinical Significance of the Quantitative Detection of DNA of Chronic Hepatitis B Virus

[目的]探讨定量检测慢性乙型肝炎(chronic hepatitis B,CHB)患者乙型肝炎病毒脱氧核糖核酸(hepatitis B virus DNA,HBV DNA)与CHB、肝脏损伤程度、HBeAg的关系。[方法]选取2010年5月至2013年3月我院收治的107例CHB患者作为研究对象,分别采用荧光定量聚合酶链式反应(FQ-PCR)、酶联免疫吸附试验(ELISA)、速率法测定所有研究对象的HBV DNA、乙型肝炎病毒e抗原(HBeAg)、丙氨酸氨基转移酶(ALT)。[结果]HBeAg阳性组患者的HBV DNA水平显著高于HBeAg阴性组患者,两者之间相比差异有统计学意义(P<0.05)。轻度、中度、重度CHB患者之间HBV DNA水平相比差异未见统计学意义(P>0.05)。CHB患者HBV DNA水平与ALT浓度之间无相关性(P>0.05)。[结论]虽然HBV DNA水平与CHB患者血清HBeAg的存在有着密切的关系,但单独检测HBV DNA难以有效地反映患者的病情严重程度以及肝细胞损伤程度,我们应对患者进行包括ALT、HBeAg等多方面的检查。

[Objective] To explore the clinical significance of quantitative detection of hepatitis B patients with chronic hepatitis B virus DNA（HBV DNA）. [Method] To select 107 cases of chronic hepatitis B patients as the research object in May 2010~March 2013 in our hospital, respectively by using fluores-cence quantitative polymerase chain reaction（FQ-PCR）, enzyme-linked immunosorbent assay（ELISA） and rate method to determine the HBV DNA,the hepatitis B virus e antigen（HBeAg） and alanine aminotransferase（ALT） of al the research object. [Results] （1）To compare with mild, moderate and severe chronic hepatitis B patients with HBV DNA level, there was no statistical significance in the difference.（2）Patients with chronic hepatitis B had no correlation between HBV DNA levels and ALT levels. （3） HBeAg positive group of patients with HBV DNA level was significantly higher than that of HBeAg negative patients;to compare the two groups, there was statistical y significant difference. [Conclusion] The severity of patients with chronic hepatitis B and liver celldamage degree have no obvious relationship with HBV DNA level, but the existence of HBeAg and HBV DNA in serum levels have a close relationship, and in the process of clinical diagnosis and treatment of chronic hepatitis B patients, we should consider several factors.