冬凌草甲素对EC9706细胞增殖、凋亡的影响

Influence of oridonin on EC9706 cell proliferation and apoptosis

目的:观察冬凌草甲素对食管鳞状细胞癌EC9706细胞增殖、凋亡的影响。方法:用MTT法检测冬凌草甲素对EC9706细胞生长的抑制作用;采用流式细胞仪技术检测冬凌草甲素对EC9706细胞周期和细胞凋亡的影响,并计算凋亡率;激光共聚焦显微镜观察不同浓度冬凌草甲素作用后EC9706细胞中游离Ca2+荧光强度的变化。结果:MTT法显示冬凌草甲素作用EC9706细胞株后,抑制率随浓度增高而增高(F=370.600,P<0.001);流式细胞仪检测结果显示,冬凌草甲素作用EC9706细胞48 h后,G1/G0期细胞均显著增多(F=24.200,P<0.001);细胞凋亡结果显示,冬凌草甲素均能明显诱导EC9706细胞凋亡(P<0.05),冬凌草甲素40μmol/L组的作用效果最佳(F=10.214、37.820,P<0.001)。不同浓度冬凌草甲素均升高了EC9706细胞内游离Ca2+荧光强度(F=24.400,P<0.001)。结论:冬凌草甲素可抑制EC9706细胞增殖、促进细胞凋亡。

Aim：To study the influence of oridonin on esophageal squamous cell carcinoma EC 9706 cell proliferation and apoptosis in vitro .Methods：MTT assay was used to detect the inhibition effect of oridonin on the growth of EC 9706;flow cytometry was applied to detect the impact of oridonin on cell cycle and apoptosis of EC 9706 , and calculated the apop-tosis rate of cells;change of Ca 2＋fluorescence intensity of EC 9706 affected by different concentrations of oridonin was ob-served by laser scanning confocal microscope .Results：MTT assay showed that the inhibition rate in the experiment group increased with the rising of concentration （F=370.600,P＆lt;0.001） after oridonin acting on EC9706; results of cell cycle detected by flow cytometry showed that the number of cell in G 0 and G1 stage increased notably after oridonin acting on EC9706 for 48 hour respectively（F=24.200,P＆lt;0.001）;results of apoptosis showed that oridonin could obviously induce the apoptosis of EC9706（P ＆lt;0.05）, and the effect was the best when the oridonin concentration was 40 μmol/L（F =10.214,37.820,P ＆lt;0.001）.Different concentrations of oridonin could increase Ca 2 ＋fluorescence intensity of EC9706,and the difference was significant （F =24.400,P ＆lt;0.001）.Conclusion： oridonin can improve the inhibition effects onproliferation and apoptosis of EC9706 cell.