特发性弱精子症患者精子中TCTE3的表达

Expression of TCTE3 in spermatozoa of idiopathic asthenozoospermic patients

目的:探讨T复合体相关睾丸表达3(TCTE3)与特发性弱精子症发生的关系。方法:采用RT-PCR和Western blot方法检测正常成年男性(正常对照)和特发性弱精子症患者各30例精子标本中TCTE3 mRNA和蛋白的表达。结果:TCTE3 mRNA和蛋白在特发性弱精子症组精子中的相对表达量均低于正常对照组(t=3.038和2.460,P=0.005和0.017)。结论:TCTE3表达的降低可能在弱精子症的发生中起着重要作用。

Aim：To explore the relationship of T-complex associated testis expressed 3（TCTE3） with idiopathic asthe-nozoospermia .Methods：The mRNA and protein expressions of TCTE 3 in 30 cases of normal adult male and 30 cases of idi-opathic asthenozoospermia semen samples were detected by using RT-PCR and Western blot .Results：The relative expres-sion levels of TCTE3 mRNA and protein in the idiopathic asthenozoospermia group were significantly lower than those in the normal control group（t=3.038 and 2.460,P=0.005 and 0.017）.Conclusion： The reduced expression of TCTE3 may play an important role in the incidence of idiopathic asthenozoospermia .