摘要
[目的]研究玉米素对蓝莓组培增殖生长的影响.[方法]以蓝莓嫩芽茎段为外植体,进行初代培养、继代增殖培养研究,探讨玉米素对蓝莓组培增殖生长的影响.[结果]WPM＋1.0～4.0 mg/L ZT＋30 g/L蔗糖＋6.8 g/L琼脂培养基组合对兔眼蓝莓腋芽的诱导分化效果较好;WPM ＋2～3 mg/L ZT＋30 g/L蔗糖＋6.8 g/L琼脂培养基的增殖培养效果较好,增殖系数大,且生长健壮.[结论]该方法探讨了玉米素对蓝莓组培增殖生长的影响,为蓝莓的快繁提供了依据.
[Objective] To study effects of corn peptide on growth of blueberry proliferation.[Method] With blueberry bud as explants,primary culture,following the research of generation of proliferation.Effects of corn peptide on growth of blueberry proliferation were discussed.[Result] The results showed that the differentiation inducing effect of WPM ＋ 1.0 to 4.0 mg/L ZT ＋ 30 g/L sucrose ＋ 6.8 g/L agar on rabbit eye blueberry bud is good; proliferation culture is better in WPM ＋2-3 mg/L ZT ＋ 30 g/L sucrose ＋6.8 g/L agar culture medium,the proliferation coefficient is large,and growing strong.[Conclusion] The effects of corn peptide on growth of blueberry proliferation were discussed,which will provide basis for blueberry rapid propagation.
出处
《安徽农业科学》
CAS
2013年第30期11961-11962,共2页
Journal of Anhui Agricultural Sciences
关键词
蓝莓
外植体
诱导分化培养
增殖培养
Blueberry
Explant
The induction and differentiation culture
Proliferation culture
