反相高效液相色谱法测定化妆品中的神经酰胺

Determination of ceramides by reversed phase high performance liquid chromatography

采用反相高效液相色谱法测定化妆品中的神经酰胺。色谱条件为：SHIMADZU Shim-pack VP-C18ODS （150 mm×4.6 mm，5 μm）色谱柱，C18ODS保护柱芯，流动相V（甲醇）︰V（异丙醇）=55︰45，流速1.0 mL·min-1，柱温30 ℃，紫外检测器检测，波长205 nm，检测池温度40 ℃。结果表明，在此色谱条件下，神经酰胺在0～5 g·L-1与相应的峰面积具有良好的线性关系（r=0.991 4），线性回归方程为A=1 466 341ρ，回收率在96.5%～101.2%。

A reversed phase high performance liquid chromatography （RP- HPLC ） method for the determination of ceramides in cosmetics was established. The sample was analyzed on a SHIMADZU Shim - pack VP- C18 ODS （150 mm ×4.6 ram,5 μm） with ClsODS guard column core and UV detector. Operating conditions were：mobile phase consisted of V（methanol） ： V（isopanol） = 55：45 at a flow rate of 1.0 mL · min - 1 ; column temperature 30 ℃ ; wavelength of the UV detector 205 nm ; detection cell temperature 40 ℃. Results showed that as the mass concentration of ceramide is in the range of 0 - 5 g · L-1, the relationship between mass concentration and the corresponding peak area shows good linearity （r = 0. 991 4 ）. The regression equation can be expressed as A = 1 466 341p and the recovery achieves 96.5% - 101.2%.