摘要
本研究对猫传染性腹膜炎病毒(FIPV)N蛋白的编码基因进行克隆和原核表达,并在纯化重组N蛋白的基础上,建立了FIPV抗体间接ELISA检测方法。研究结果显示,该重组纯化的N蛋白具有良好的抗原反应性,可用于FIPV阳性血清的筛查,为我国出入境检疫部门监控FIPV疫情提供技术支撑。
In this study,the gene of nucleocapsid protein（N protein）of feline infectious peritonitis virus（FIPV） was cloned and expressed in Escherichia coli. And an indirect ELISA method was established with the purified recombi-nant N protein. The results showed this recombinant N protein had perfect antigen reactivity of FIPV and could be used to screen FIPV positive serum,and the study was helpful to monitor the FIPV infection status.
出处
《中国动物检疫》
CAS
2014年第2期67-70,共4页
China Animal Health Inspection
基金
上海市科委科研项目(课题编号11140901100)
关键词
猫传染性腹膜炎病毒
核衣壳蛋白
基因克隆和表达
间接ELISA
feline infectious peritonitis virus
nucleocapsid protein
gene cloning and expression
indirect ELISA
