常压低氧耐力训练对肥胖大鼠Visfatin水平的影响

Effects of Hypoxic Endurance Training on the Visfatin Levels in Obesity Rats

目的：通过建立肥胖大鼠低氧训练模型，观察血清Visfatin含量和脂肪组织Visfatin基因表达水平，探讨低氧训练对肥胖大鼠Visfatin的影响。方法：单纯性肥胖大鼠建模成功后，筛选130只随机分为13组：对照0周组，低氧安静1、2、3、4周组，常氧训练1、2、3、4周组，低氧训练1、2、3、4周组。低氧环境模拟海拔3500m（氧浓度13．6％）；常氧和低氧训练组分别以25m／min、20m／min进行跑台训练，各训练组持续运动1h／d、6d／w、1～4周。采用ELISA法测试血清Visfatin含量，荧光定量PCR法测试附睾脂肪VisfainmRNA相对表达量。结果：1）肥胖大鼠0周时血清Visfatin含量（50．095±9．092μg／L）显著高于其他各周（P〈0．01），常氧训练第1、2、3、4周时分别为17．856±3．596μg／L、27．002±10．475μg／L、26．866±7．107μg／L、36．385±5．455μg／L，低氧安静组第1、2、3、4周时分别为22．382±6．356μg／L、24．662±6．654μg／L、25．764±9．382μg／L、34．504±6．946μg／L，低氧训练组第1、2、3、4周时分别为17．227±5．106μg／L、18．360±5．181μg／L。15．065±4．466μg／L、16．689±7．728μg／L；常氧训练和低氧安静组，其变化趋势一致，第1周显著性下降（P〈0．01），随后回升；而低氧训练组一直保持在低水平，后3周均显著低于其他两组（P〈0．01）。2）肥胖大鼠0周时脂肪组织VisfatinmRNA相对表达量为0．0658±0．0263，常氧训练组第1、2、3、4周时分别为0．0569±0．0237、0．0499±0．0130、0．0653±0．0288、0．1071±0．0437，第4周时显著高于第1、2、3周时水平（P〈0．01）；低氧安静组第1、2、3、4周时分别为0．0883±0．0442、0．0990±0．0570、0．0507±0．0178、0．0883±0．0312；低氧训练组第1、2、3、4周时则表现出下降的趋势，分别为0．1102±0．0368、0．0845±0．0238、0．0679±0．0245、0．0668±0．0298，低氧训练第4周时其相对表达量低于常氧训练和低氧安静组，与常氧训练组比较差异显著（P〈O．05）。结论：1）低氧训练控制肥胖大鼠血清Visfatin水平较常氧训练和低氧安静更为有效；2）与常氧训练和低氧安静比较，低氧训练下调脂肪组织Visfatin基因表达作用更明显。

This paper is aimed to discuss the effects of hypoxic endurance training on the Visfa- tin levels in obesity rats by observing the serum Visfatin level and the gene expression of Vis- latin mRNA. Methods： 130 obesity rats were divided into 13 groups as the followings：control 0 week group, hypoxic control 1,2,3,4 week groups, normoxic 1,2,3,4 week training groups and hypoxie 1,2,3,4 week training groups. All hypoxic training groups stayed in the environ ment with 13.6% oxygen concentration. The treadmill training was applied with 25m / min in normoxic training groups and 20 m/rain in hypoxic training groups. The rats were trained for 1h/d, 5d/w, 1~4 weeks respectively. The Visfatin mRNA genes expressions in fat tissue of epididymis were measured by quantitative fluorescent PCR, and the serum Visfatin levels were measured by ELISA. Results ： 1） The serum Visfatin level of 0 week reaches 50. 095 ±9. 092μg/L and all the other groups ＇ are significantly blow to its （P〈0.01）. There are the same trend in hypoxic control groups and normoxic training groups with first decrease and then in- crease variation. While it always keeping low level in hypoxic training groups. 2）The Visfatin mRNA gene expression of 0 week in epididymis fat tissue reaches 0.0658 ± 0.0263. The Vis- Satin gene expression in normoxic training groups has the same trend as the serum Visfatin lev- els. Although there is the trend with first up regulating and then down regulating in hypoxic control groups and hypoxic training groups, the gene expression of hypoxic 4 week training group is significantly down regulated compared to hypoxic training 1 week group, hypoxic con- trol and normoxic training 4 week groups （P 〈0. 01 ）. Conclusion ： 1 ） Hypoxic training has more efficency than hypoxic control and normoxic training for decreasing the serum Visfatinlevels. 2）Hypoxic training also has more efficiency than hypoxic control and normoxic training for down regulating the Visfatin mRNA gene expression.