A1蛋白与前列腺特异性抗原在前列腺癌与前列腺增生疾病中联合检测的意义

The clinical value of combined detection of A1 protein and prostate specific antigen in prostate cancer and prostatic hyperplasia

目的选取50—70岁之间的前列腺增生、前列腺癌患者和健康对照者，对其血液中A1蛋白的表达与PSA的表达水平进行比较分析，为前列腺增生和前列腺癌的临床鉴别诊断提供依据。方法采用电化学发光仪检测PSA浓度，采用反转录酶一聚合酶链锁反应（RT—PCR）技术和蛋白免疫印迹法（western．blot）检测20例前列腺癌患者、20例前列腺增生患者和20例正常者新鲜前列腺组织中A1mRNA和蛋白的表达情况，探讨A1蛋白表达与前列腺增生和前列腺癌中PSA表达水平的关系。结果RT—PCR与westem．blot检测结果一致，A1蛋白在前列腺增生组织和前列腺癌组织中均有阳性表达，前列腺癌组织中A1蛋白阳性表达明显高于前列腺增生组织，有显著性差异（P〈0．01）。A1蛋白表达的阳性率与PSA的浓度相关。结论A1蛋白阳性表达程度与前列腺病变程度相关，A1蛋白在细胞凋亡调节中起着重要的作用，其过度表达在前列腺癌的发生、发展中扮演着重要的角色。联合检测A1和PSA的表达变化对判断前列腺癌的生物学行为有参考价值。

Objective This study aims to investigate the clinical value of combined detection of PSA and A1 to dif- ferentiate prostate cancer from bengin prostatic hyperplasia. Methods The serum PSA levels were detected by electro chemiluminescence, The expression of A1 in the prostate cancer and benign prostatic hyperplasia was detected by RT-PCR and western-blotting. The relationship of expression of A1 protein with PSA was analyzed. Results The expression of A1 protein in patients with prostate cancer was significantly lower than that in patients with benign prostatic hyperplasia and healthy persons（P〈O. 01 ）. The expression of A1 protein was related with the concentration of PSA. Conclusions A1 protein is correlated with the modulation of proliferation in the development of prostate cancer. The expression of A1 protein plays an important role in the development of prostate cancer. The combined detection of PSA and A1 protein is helpful in the diagnosis of prostate cancer.