番茄红素对高糖所致内皮祖细胞功能障碍的保护作用及机制初探

Effect of Lycopene on the Function and Mechanism of Human Peripheral Blood Endothelial Progenitor Cells Cultivated in High Concentration Glucose

本文阐述了番茄红素对高糖所致人外周血内皮祖细胞(endothelial progenitor cells,EPCs)功能障碍的影响,并探讨了其可能机制。EPCs分为空白对照组、高糖组、番茄红素10、30、50μg/mL低、中、高剂量和信号阻断剂(1μmol/L SB203580)组,用33 mmol/L葡萄糖诱导EPCs损伤,分别采用MTT比色法、黏附能力测定实验、改良的Boyden小室检测EPCs黏附、迁移能力,Matrigel成血管网实验评价体外小管形成能力,Western-blot检测磷酸化和非磷酸化p38 MAPK蛋白表达量。实验表明番茄红素可改善高糖环境下EPCs黏附、迁移和体外小管形成能力,其机制可能与番茄红素抑制EPCs中p38 MAPK激活有关。

The objectives of this study were to investigate the effects of lyeopene on migratory, adhesive, tube formation capacity and the activity of p38 mitogen-activated protein kinase （p38 MAPK） of endothelial progenitor ceils（EPCs） with high concentration of glucose and to explore the protection mechanism of EPCs of human peripheral blood by lyco- pene. Mononuclear cells （MNCs） were isolated from endothelial progenitor cells of human peripheral blood by Ficoll density gradient centrifugation, cultured and identified. The third generation of EPCs was incubated with high concentra- tion glucose （33 mmol/L） and with lycopene plus high glucose. EPCs migration was assessed with MTT assay and modi- fied Boyden chamber assay. EPCs adhesion assay was performed by replating those on fibronectin-coated dishes, and then adherent cells were counted. Tube formation activity was assayed by matrigel network formation assay. Western blot assay was used to analyze phosphorylated and non-phosphorylated p-38 mitogen activated protein kinases （MAPKs） protein expression. The results showed high concentration glucose attenuated migratory, adhesive, capacity of EPCs and increased phosphorylation of p38 MAP kinase. Lycopene improved function of EPCs to high concentration glucose through inhibi- ting p38 MAP kinase pathway,which was one of mechanisms responsible for protecting EPCs.