素馨花提取物橄榄苦苷在大鼠体内药代动力学研究

Study on Pharmacokineics of Oleuropein from Jasminum officinale L.var.grandiflorum in Rats

本文对素馨花中提取纯化所得橄榄苦苷在大鼠体内的药代动力学过程进行研究。将雄性Wistar大鼠随机分为橄榄苦苷100、200、400 mg/kg剂量组,灌胃(i.g.)给药后不同时间采血,离心,血浆经甲醇-乙腈沉淀蛋白后,HPLC测定不同时间点大鼠血浆中橄榄苦苷的含量。各给药组平均血药浓度-时间数据应用3P97软件进行药代动力学参数分析,组间药动学参数用SPSS 17.0进行统计分析。结果表明,橄榄苦苷在大鼠体内的药代动力学过程符合二室模型,低、中、高剂量组的t1/2、CL/F、AUC差异具有显著的统计学意义,其中t1/2、CL/F随给药剂量的增大而增大;AUC随剂量的增大,呈先减小后增大的趋势,橄榄苦苷在大鼠体内的药代动力学呈非线性过程。

The objective of this study was to study the pharmacokinetics of oleuropein extracted form Jasminum officinale L. var. grandiflorum in healthy rats. Male Wistar rats were randomly divided into 100,200,400 mg/kg of oleuropein group, The rats were intragastric administrated （i. g. ） at different time. After the drug administration, blood sampling, centrifugal, plasma protein precipitation with methanol and acetonitrile were carried out. The concentration of oleuropein in blood serum was determined by high performance liquid chromatography （HPLC） at different time points. The average blood oleuropein concentration-time data set was used to analyze the pharmacokinetic parameters by 3P97 software. Tile pharmacokinetic parameters between different groups were applied to SPSS 17.0 software for statistical analysis. The re- sults showed that the pharmacokinetics of oleuropein in rats was fitted with two compartment model The t1/2, CL/F, AUC of low, medium and high dose sample group had significant difference. The tt/2 and CL/F increased with the increasing of dose,while the AUC decreased firstly and then increased with increasing of dose. These results proved that the pharma- cokinetics of oleuropein in rats were a non-linear process.