摘要
采用硅胶柱、反相硅胶柱和凝胶柱等色谱技术,从北极真菌Eutypella sp.的菌体发酵液提取物中分离纯化得到2个化合物,通过1H和13C NMR、2D NMR等分析方法,并比较相关文献,鉴定这两个化合物为libertellenone C(1)和libertellenone A(2)。测试了化合物1和2对各种癌细胞株的细胞毒活性,其中化合物1对胰腺癌SW1990和胶质瘤U251有一定的细胞毒活性,此外化合物2对稻瘟霉有抑制活性。
Two pimarane diterpenes were isolated from the fermentation broth of the Arctic-derived fungus Eutypella sp. D-1 by various chromatographic methods. On tile basis of 1 H, 13 C and 2D NMR spectral data, compounds 1 and 2 were i- dentified as lisbertellenone C （1） and libertellenone A （2）. The potential anticancer activity and antifungal activity a- gainst eonidia of Pyricularia oryzae of compounds 1 and 2 were evaluated. Compound 1 showed weak eytotoxicities a- gainst two tumor cell lines SW-1990 and U-251 ,compound 2 exhibited antifungal activity against conidia of Pyricularia oryzae with the MIC of 18.8 μg/mL.
出处
《天然产物研究与开发》
CAS
CSCD
北大核心
2014年第1期56-59,共4页
Natural Product Research and Development
基金
国家863项目子课题(SS2012AA09160703)
广东省公益性项目(GD2012-D01-001)
国家自然科学基金项目(41306197)
